Agilent 8000 Dissolution Sampling Station Operator’s Manual Agilent Technologies
Notices © Agilent Technologies, Inc. 2014 Warranty No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws. The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions.
Contents Figures 1 7 Tables 9 Safety 11 Electrical Hazards 12 Warning 13 Caution 13 Note 13 Information Symbols 2 Introduction 14 15 8000 with Peristaltic Pump 17 Conventions Used in this Manual Serial Number Format 3 Setting Up the 8000 17 18 19 Unpacking of 8000 and Components 20 Unpacking Your 8000 21 Unpacking / Setup of 8000 & Peristaltic Pump 22 Unpacking / Setup of 8000 & Syringe Pump 26 Unpacking / Setup of 8000, Syringe Pump, and Filter Changer 29 Setup of 8000 and Components 34 In
Contents Installing the Media Rinse Reservoir 36 Installing and Connecting the Autocalibration Block (Peristaltic Pump only) 37 Installing the RM Module 38 Loading Filters (Filter Changer only) 40 Changing Filter Types (Filter Changer Only) 40 Hidden Key Functions 4 Operating the 8000 41 45 Operating the 8000 with Apparatus 1 / 2 8000 Keypad Options 46 8000 Menu Options - Apparatus 1 / 2 Creating a Sample Program 61 Starting a 8000 Program 66 46 48 Operating the 8000 with Apparatus 3 / 7 72 8000 Keypa
Contents Syringe and Seal Replacement 112 Cleaning the Syringes 113 System Cleaning Procedure with Syringe Pump and Filter Changer (optional) 115 Cleaning the Filter Changer Drip Tray 117 Cleaning the Media Replacement Pump 117 Report Center Impact Printer 119 Installing the Cartridge Ribbon 119 Replacing a Paper Roll 120 Toggling Your Printer Online 121 Printer Self Test 121 Printer Configuration 122 Obtaining Warranty and Other Services Index 8000 Operator’s Manual 123 125 5
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Figures Figure 1. Agilent 8000 Dissolution Sampling Station 21 Figure 2. Peristaltic Pump 22 Figure 3. Cable Connections for 8000, Peristaltic Pump, and VK 7000 / 7010 24 Figure 4. Resident Probe 25 Figure 5. 806 Syringe Pump 26 Figure 6. Cable Connections for 8000, Syringe Pump, and VK 7000 / 7010 27 Figure 7. Y Cable 5075-0446 and Adapter 5075-0852 28 Figure 8. 808 Filter Changer 30 Figure 9. Cable Connections for 8000, Syringe Pump, Filter Changer, and VK 7000 / 7010 31 Figure 10.
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Tables Table 1. Table 2. Table 3. Table 4. Table 5. Table 6. Table 7. Table 8. Table 9. Hidden Key Functions 41 Front Panel Options (Apparatus 1 / 2) 47 Main Menu Options (Apparatus 1 / 2) 49 Set Clock and Alarms Options (Apparatus 1 / 2) 51 Disso Parameters Options (Apparatus 1 / 2) 53 Set Syringe Pump Parameters (Apparatus 1 / 2) 55 Disso Control/Monitor Options (Apparatus 1 / 2) 57 Manual Operations Options (Apparatus 1 / 2) 58 Operate Syringe/Filter Changer Options (Apparatus 1 / 2) 59 Table 10.
Tables Table 25. Table 26. Table 27. Table 28. Table 29. Table 30. 10 Manual Sampling Options 95 Volume Accuracy Parameter Options Print Selections Options 104 Program Auto Clean Variables 110 Disso Parameters.
Agilent 8000 Dissolution Sampling Station Operator’s Manual 1 Safety Electrical Hazards 12 The Agilent 8000 has been designed and tested so that when used properly you have an accurate, fast, flexible, and safe instrument. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Operation of a Agilent 8000 involves the use of aqueous liquids and various pharmaceutical dosage forms.
1 Safety Electrical Hazards The 8000 contains electrical circuits, devices, and components operating at dangerous voltages. Contact with these circuits, devices, and components can cause death, serious injury, or painful electric shock. Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Agilent-trained, Agilent-qualified, or Agilent-authorized service engineers.
Safety 1 Warning WA RNING A ‘Warning’ message appears in the manual when failure to observe instructions or precautions could result in death or injury. Read all warnings and cautions carefully and observe them at all times. Caution CAUTION A ‘Caution’ message appears in the manual when failure to observe instructions could result in damage to equipment (Agilent supplied and / or other associated equipment). Note NOT E A ‘Note’ appears in the manual to give advice or information.
1 Safety Information Symbols I 0 Switches main power on Switches main power off Indicates single-phase alternating current Indicates the product complies with the requirements of one or more European Union (EU) directives. Indicates specific equipment meets standards of safety. These products are safe for use in the workplace for North America. Indicates that this product must not be disposed of as unsorted municipal waste.
Agilent 8000 Dissolution Sampling Station Operator’s Manual 2 Introduction 8000 with Peristaltic Pump 17 Conventions Used in this Manual Serial Number Format 18 17 Agilent Technologies 15
2 Introduction The 8000 Dissolution Sampling Station provides an unattended solution for automated sampling from a dissolution apparatus. The instrument may be configured to control any instrument setup with a USP apparatus 1 (Baskets), 2 (Paddles), 3 (Reciprocating Cylinder), 5 (Paddle over Disk), 6 (Rotating Cylinder), or 7 (Reciprocating Holder) offered by Agilent Technologies, Inc. The firmware can store up to 15 different sampling programs.
Introduction 2 8000 with Peristaltic Pump The 8000, when used in conjunction with the peristaltic pump, is capable of performing unattended sampling from a dissolution apparatus. All parameters of operation are controlled by the 8000. Sampling is carried out automatically and takes into account all relevant variables in the sample flow path such as tubing length, tension, and diameter. The volume calibration may be performed manually or automatically using an autocalibration block supplied with the unit.
2 Introduction Serial Number Format The serial number contains 10 characters and follows this syntax: CC1234xxxx Syntax Code 18 Meaning Description CC Country of origin 2 alpha characters matching the required trade designation for the country of origin 12 Year of manufacture ‘09’ for 2009, ‘10’ for 2010, etc.
Agilent 8000 Dissolution Sampling Station Operator’s Manual 3 Setting Up the 8000 Unpacking of 8000 and Components Setup of 8000 and Components 34 Hidden Key Functions 41 20 Agilent Technologies 19
3 Setting Up the 8000 Unpacking of 8000 and Components Check the contents for any damages due to shipping. The packaging used to ship your equipment ensures that damage rarely occurs, but if it does, contact both the carrier who delivered the instrument and the Dissolution Systems Service Department. Though claims for damage should be filed with the carrier, we will be glad to help you file a claim and get you up and running as quickly as possible.
Setting Up the 8000 3 Unpacking Your 8000 Figure 1 Agilent 8000 Dissolution Sampling Station The 8000 is shipped in foam-filled cartons. The number of cartons may vary depending on your configuration. 1 Carefully remove the 8000 and accessory kit(s) from their shipping cartons. Remove all cushioning material and tape. 2 Place the 8000 on a clear, dry, level section of the bench top close to the dissolution apparatus. The preferred placement of the 8000 is on the right side of the dissolution apparatus.
3 Setting Up the 8000 Unpacking / Setup of 8000 & Peristaltic Pump Environmental Requirements for Peristaltic Pump Installation • Altitude: max 2000m; • Humidity: max relative humidity 80% for temperatures up to 31 ºC decreasing linearly to 50% relative humidity at 40 ºC • Indoor use only • Temperature: 5 ºC to 40 ºC • Main supply voltage fluctuations are not to exceed ± 10% of the nominal supply voltage • Operating conditions: 3 mins ON Max; 5 mins OFF Min Figure 2 Peristaltic Pump Installation 1 Care
Setting Up the 8000 3 5 Loosely fit the peristaltic pump tubing along the inside groove of the first cartridge, locking the tubing clips on the outside notches. NOT E Ensure that all pump cartridges are installed. It is not necessary to install tubing on any unused positions. 6 Refit the cartridge to the pump by hooking the clip on the bottom left of the cartridge into the groove on the left side of the pump.
3 Setting Up the 8000 apparatus: Figure 3 Cable Connections for 8000, Peristaltic Pump, and VK 7000 / 7010 11 Connect the five-pin cable (#2, P/N 5075-0033) between the jack on the 8000 rear panel labeled PUMP and the jack on the peristaltic pump rear panel labeled PUMP CONTROL. 12 If the system configuration includes the 705-DS, connect the 9-to-25 pin cable to the RS232 port on the dissolution apparatus and the RS232 COMPUTER port on the 8000 rear panel.
Setting Up the 8000 3 13 The 8000 is shipped with all sample tubing attached to the valves inside the dispensing arm. NOT E Do not remove the nylon tie wraps securing the tubing into bundles. The direction of the peristaltic pump can be set via a switch on its rear panel. It does not matter at this point to which side the bundles go, as long as all lines in the bundle from the sampling cannulas are connected to one side, and all lines in the bundle from the valve inlets to the other.
3 Setting Up the 8000 16 Connect the remaining length of tubing coming from the top positions of the 8000 solenoid valves to the return lines on the dissolution apparatus. Ensure each line is attached to its identical color. WA RNING Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger-tight. Overtightening will damage the fittings. 17 For a system that includes a 705-DS, connect the return lines to the return cannulas of the resident probes.
Setting Up the 8000 3 3 Place the 806 Syringe Pump on a clean, dry, level section of the bench top between the 8000 and the dissolution apparatus. 4 Refer to the following example diagram to correctly make cable connections for the 8000, syringe pump, and dissolution apparatus: Figure 6 Cable Connections for 8000, Syringe Pump, and VK 7000 / 7010 5 Connect the single end of the Y cable (#1, P/N 5075-0446) to the RS232 port on the syringe pump.
3 Setting Up the 8000 8 A system configuration that includes a non-Agilent dissolution apparatus, requires use of an adapter (P/N 5075-0852, see following Figure). This adapter should be attached to the #2 port of the Y-cable in place of the cable connected to the dissolution apparatus. Figure 7 Y Cable 5075-0446 and Adapter 5075-0852 9 The 8000 is shipped with all sample tubing attached to the valves inside the dispensing arm. NOT E Do not remove the nylon tie wraps securing the tubing into bundles.
Setting Up the 8000 3 the 8000 solenoid valves. Ensure each line is attached to its identical color. 12 Connect the remaining length of tubing from the top positions of the 8000 solenoid valves to the return lines on the dissolution apparatus. Ensure each line is attached to its identical color. WA RNING Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger-tight. Overtightening will damage the fittings.
3 Setting Up the 8000 Installation Figure 8 808 Filter Changer The filter changer is shipped in individual packaging. 1 Carefully lift the unit out of the box. 2 Remove any cushioning material. 3 Place the unit on a clear, dry, level section of the bench top between the 8000 and the syringe pump.
Setting Up the 8000 3 4 Refer to the following example diagram to correctly make cable connections for the 8000, syringe pump, filter changer, and dissolution apparatus: Figure 9 Cable Connections for 8000, Syringe Pump, Filter Changer, and VK 7000 / 7010 5 Connect the single end of a Y cable (#1, P/N 5075-0446) to the RS232 port on the filter changer.
3 Setting Up the 8000 between the remaining extension of the Y cable attached to the syringe pump and the RS232 port on the dissolution apparatus. 10 A system configuration that includes a non-Agilent dissolution apparatus, requires use of an adapter (P/N 5075-0852, see following Figure). This adapter should be attached to the #2 port of the Y-cable in place of the cable connected to the dissolution apparatus.
Setting Up the 8000 3 starting with Position 1. resident probe housing sample cannula Figure 11 Resident Probe Housing and Sample Cannula 13 Connect a separate length of tubing from the right port (labeled OUT) of each syringe to the top inlet ports of the filter changer starting with the port closest to the carousel. 14 Connect another separate length of tubing lines from the lower side ports of the filter changer to the tubing coming from the left positions of the 8000 solenoid valves.
3 Setting Up the 8000 Setup of 8000 and Components Installing 8000 Full Flow Filters The 8000 has been supplied with 10-, 35-, and 70-micron Full Flow Filters for use with the sampling cannulas. In theory, dissolution continues until the samples are filtered. The 8000 allows filtering at the instant a sample is taken with the use of the Full Flow Filters. Some drug excipients may clog the Full Flow filters reducing their performance. Filters may need to be changed during a run only in extreme cases.
Setting Up the 8000 3 • If your system configuration includes the syringe pump, the power receptacle, and power switch are located on the left side of the unit. Connect the power cord and turn on the syringe pump. • If your system configuration includes the filter changer, the power receptacle, and power switch are located on the rear of the unit. Connect the power cord and turn on the filter changer. The 8000 has a dual voltage (115 V / 230 V) option on the power entry module.
3 Setting Up the 8000 Installing the Sample Tray Place the supplied sample tray on top of the unit. The sample tray has four grommeted corner holes which fit over the four locating pins. WA RNING The sample tray must be securely mounted on the locating pins. Otherwise, damage can occur to the sampling needles and drive mechanism. Installing the Media Rinse Reservoir The media rinse reservoir fits in the rear of the unit between the metal brackets.
Setting Up the 8000 3 If your system configuration includes the peristaltic pump, complete the steps under “Installing and Connecting the Autocalibration Block (Peristaltic Pump only)” on page 37. Installing and Connecting the Autocalibration Block (Peristaltic Pump only) 1 With the dispensing arm forward, insert the autocalibration block behind the media rinse reservoir. Ensure the RS232 port is positioned to the right for a 6-row configuration and toward the back for a 12-row configuration.
3 Setting Up the 8000 Installing the RM Module Internal Unit Pump 1 Pump 1 Pump 2 Pump 2 Figure 12 Internal Media Replacement Module 1 Attach a length of 1/2-inch diameter tubing to the IN fitting of pump 1 (the top pump). 2 Place the free end in one of the unstirred vessels containing medium or in the receptacle being used to hold the replacement medium. 3 If desired, attach a clip to regulate flow speed on this length of tubing.
Setting Up the 8000 3 External Unit Figure 13 External Media Replacement Module 1 Remove the cover housing the RM chamber from the side of the 8000. 2 Detach the RM chamber. 3 Insert the end of the tubing attached to the IN fitting on the pump into the plastic RM chamber. 4 Slide a tubing clamp on a length of tubing. 5 Connect one end of the tubing containing the clamp to the OUT fitting on the pump. Connect the other end to the IN fitting of the RM chamber.
3 Setting Up the 8000 Loading Filters (Filter Changer only) 1 Choose a tube of filters. Although you can load up to eight tubes of filters, only one tube is necessary for the filter changer to function properly. 2 Remove the cap from the end of the tube to expose the long, thin exit nozzle of the end filter. 3 Insert the tube, with the open end down, into the metal filter assembly in the filter changer carousel. Pivot the tube until it slides vertically into the white stabilizer slot.
Setting Up the 8000 3 10 Load the new tubes of filters into the carousel of the filter changer. See “Loading Filters (Filter Changer only)” on page 40. 11 Select option 3, Change Filters. 12 Enter 8 as the number of filters to change and press ENTER. The filters expel from the filter changer. 13 When the filter changer stops expelling filters, select option 3, Change Filters, again. 14 Enter 1 as the number of filters to change and press ENTER.
3 Setting Up the 8000 Table 1 Hidden Key Functions Key Sequence Function MENU > 0 > D Use this key sequence to enable or disable the multiple sample option. The asterisk (*) displays next to the current selection. Note: For use with APP 3/7 (Apparatus 3 / 7) systems only. MENU > 0 > E Use this key sequence to select the printer type. Ensure the asterisk (*) displays next to option 1, Impact. If not, select option 1 to enable the impact printer.
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3 44 Setting Up the 8000 8000 Operator’s Manual
Agilent 8000 Dissolution Sampling Station Operator’s Manual 4 Operating the 8000 Operating the 8000 with Apparatus 1 / 2 46 Operating the 8000 with Apparatus 3 / 7 72 Manual Sample 95 Volumetric Calibration (Peristaltic Pump only) 97 Daisy Chaining 102 Printer Operation and Communications 103 Agilent Technologies 45
4 Operating the 8000 Operating the 8000 with Apparatus 1 / 2 8000 Keypad Options The keypad on the 8000 is similar to a personal computer keyboard. For example, it has a SPACE key, an ENTER key, and a SHIFT key. These keys function exactly like their counterparts on a personal computer or typewriter keyboard. See Figure 14, “8000 Keypad,” below. A discussion about each of the main function keys follows.
Operating the 8000 4 The front panel options include the following: Table 2 Front Panel Options (Apparatus 1 / 2) Option Function ESC Press ESC to stop execution of a running program or calibration routine. Press ESC to move back one level to the previous menu or selection when moving through the multi-level menu. Note: Be very careful when using ESC while a program is running.
4 Operating the 8000 Table 2 Front Panel Options (Apparatus 1 / 2) Option Function PUMP OFF PUMP OFF, PUMP FWD, and PUMP REV work exclusively with the Agilent peristaltic pump. When the syringe pump is connected, they are disabled. PUMP FWD PUMP REV CLEAN SYTEM Use this option to keep the valves, needles, and fluid lines clean. LOCK KBRD Press LOCK KBRD to lock the keypad. Press LOCK KBRD again to unlock the keypad. 8000 Menu Options - Apparatus 1 / 2 Main Menu From the Ready screen, press MENU.
Operating the 8000 4 Following is a description of the Main Menu screen options: Table 3 Main Menu Options (Apparatus 1 / 2) Option Function 1 Set Clock/Alarms Use this option to set the clock and alarms. If your system configuration includes the syringe pump, the start output delay option also displays on this screen. See “Main Menu Option 1 - Set Clock/Alarms” on page 51. 2 Set DISSO Use this option to set the DISSO parameters. See “Main Menu Option 2 - Set DISSO” on page 53.
4 Operating the 8000 Table 3 Main Menu Options (Apparatus 1 / 2) Option Function 4 Manual Prime Vol. (syringe pump) If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system. Select option 4, Manual Prime Vol. Enter a value for the priming volume and press ENTER. The Main Menu displays.
Operating the 8000 4 Main Menu Option 1 - Set Clock/Alarms From the Main Menu, select option 1, Set Clock/Alarms. The Set Clock and Alarms screen displays. ***SET CLOCK AND ALARMS*** 1 SET CLOCK 2 TIMER ALARM 3 ROW COMPL ALARM 4 CALIB. COMPL ALARM 5 PRG COMPL ALARM 6 START OUTPUT DELAY Following is a description of the Set Clock and Alarms screen options: Table 4 Set Clock and Alarms Options (Apparatus 1 / 2) Option Function 1 Set Clock Select option 1, Set Clock.
4 Operating the 8000 Table 4 Set Clock and Alarms Options (Apparatus 1 / 2) Option Function 4 Calib. Compl Alarm Use this option to set an alarm to sound when autocalibration is complete and all lines are purged. Select option 4, Calib. Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays. Press any key to silence the alarm after it sounds. Note: This option is only available if your system configuration includes the peristaltic pump.
Operating the 8000 4 Main Menu Option 2 - Set DISSO From the Main Menu, select option 2, Set DISSO. Enter a program number and press ENTER. The DISSO Parameters screen displays. ***DISSO PARAMETERS*** 4 SET BATH TEMP (VESSEL TEMP) 1 SET SPINDLE RPM 5 COM PORT ID 2 FINAL SPIN LENGTH 6 DDM OR BASKET 3 INI/FINAL VESSEL TEMP 7 PRINT FREQ NOT E DISSO applies to all dissolution apparatus.
4 Operating the 8000 Table 5 Disso Parameters Options (Apparatus 1 / 2) Option Function 4 Set Bath Temp If your system configuration includes a dissolution apparatus with a water bath, select option 4, Set Bath Temp. If your system configuration includes a dissolution apparatus with Direct Vessel Heating (DVH), select option 4, Vessel Temp. 4 Vessel Temp Select option 1, Manual, to set the water bath / vessel temperature.
Operating the 8000 4 Once all values have been entered, press ESC. A confirmation screen displays. Press Y to send the newly entered configuration to the dissolution apparatus and override the existing settings. Main Menu Option 3 - Set Syringe Pump This section is applicable only to systems including a syringe pump. NOT E Ensure the syringe pump is enabled prior to setting the syringe pump parameters (see MENU > 0 > P under “Hidden Key Functions” on page 53).
4 Operating the 8000 Table 6 Set Syringe Pump Parameters (Apparatus 1 / 2) Option Function 3 Prime Loss Volume Use this option to configure the prime loss volume. Prime loss volume compensates for the volume of medium used to fill the tubing between the sampling cannulas and sampling valves during the priming cycle. When sampling, an amount of sample equal to the prime loss volume flows past the valves in the 8000 before the valves open to collect sample.
Operating the 8000 4 Main Menu Option 6 - Control DISSO From the Main Menu, select option 6, Control DISSO. The DISSO Control/Monitor screen displays. ***DISSO CONTROL/MONITOR*** 1 RUN 2 STOP 3 LIFT 4 LOWER 5 STILL SET RPM: 100.0 ACTUAL RPM: 000.0 SET BATH TEMP: 37.5 ACTUAL TEMP: 37.
4 Operating the 8000 The following Manual Operations screen displays when your system configuration includes the peristaltic pump: ***MANUAL OPERATIONS*** 1 LIFT VALVES 2 LOWER VALVES 3 OPEN/CLOSE VALVES 4 GOTO ROWS 5 TURN ON (OFF) R-M PUMP The following Manual Operations screen displays when your system configuration includes the syringe pump with or without the filter changer: ***MANUAL OPERATIONS*** 1 LIFT VALVES 2 LOWER VALVES 3 OPEN/CLOSE VALVES 4 GOTO ROWS 5 TURN ON (OFF) R-M PUMP 6 SYRING
Operating the 8000 Table 8 4 Manual Operations Options (Apparatus 1 / 2) Option Function 5 Turn On (Off) R-M Pump Select option 5, Turn On (Off) R-M Pump, to toggle the replacement media option off and on, if installed. 6 Syringe/Filter (syringe pump only) Use this option to manually control the syringe pump and filter changer as applicable. See “Manual Operation Option 6 - Syringe/Filter” below.
4 Operating the 8000 Table 9 Operate Syringe/Filter Changer Options (Apparatus 1 / 2) Option Function 3 Syringe Fill Use this option to draw a specific amount of medium into the syringes. Ensure the valve is in the input position before drawing the medium (“1 Valve to Input” on page 59). Select option 3, Syringe Fill. Enter the desired percentage of each syringe to fill and press ENTER. The syringes draw the specified amount of medium.
Operating the 8000 4 Creating a Sample Program NOT E Programs are not write-protected. Check with other users before proceeding. To program the dissolution apparatus with the 8000, you must enable external control on the dissolution apparatus and log out. For more information, see the applicable Operator’s Manual for your dissolution apparatus. 1 From the Ready screen, press PROG to program the 8000. 2 Enter a program number and press ENTER. The Program Variables screen displays, as shown on page 61.
4 Operating the 8000 The following Program Variables screen displays when your system configuration includes the syringe pump with or without the filter changer: NOT E ***PROGRAM VARIABLES*** 4 HEADERS 1 SET DISSO PARAMETERS 5 PRIME VOLUME 2 SAMPLE TIME POINTS 6 PURGE VOLUME 3 SAMPLE VOLUME 7 FILTER CHG or R-M If your system configuration includes the syringe pump and filter changer, option 7 displays as Filter Chg. See “7 Filter Chg (syringe pump and filter changer)” on page 64.
Operating the 8000 4 Table 10 Program Variables Options (Apparatus 1 / 2) Option Function 4 Headers Select option 4, Headers. The Batch # Selections screen displays. For each option, enter the desired information and press ENTER. Press ESC to return to the Program Variables screen. Note: The Batch # Selections screen displays options for the report header information. The print field accepts ten characters. Characters can be letters, numbers, special characters or spaces.
4 Operating the 8000 Table 10 Program Variables Options (Apparatus 1 / 2) Option Function 6 Purge Volume (syringe pump) If your system configuration includes the syringe pump, use this option to set a purge volume that ensures all stranded medium is properly expelled. Select option 6, Purge Volume. Enter a value for the purge volume that ensures at least two strokes of the syringe plunger and press ENTER. The Program Variables screen displays.
Operating the 8000 4 Program Option 2 - Sample Time Points From the Program Variables screen, select option 2, Sample Time Points. The following screen displays: SAMPLE TIME POINT: 000:02 ENTER hhh:mm UP TO 999:59 HOURS PROG #: 1 ROW: 0 VOLUME: 5ml TIME:01/01/12 09:37:14 NOT E ELAPSE:000:00:00 This screen accepts sampling times and saves them for execution when the program is run. Time points are requested in order of execution starting with collection Row 0 on the sample tray.
4 Operating the 8000 • If a shorter sample time point than the previous one is entered for a given row, the row number will not increment. Times must increase from one row to the next. • To shorten a saved program, enter 000:00 for the first sample time point you wish to discard. All successive time points are eliminated. • Press ESC to save all new values and return to the Program Variables screen.
Operating the 8000 4 3 To run a single program, select option 1. NOT E If more than ten rows are required to collect samples, linking a program allows you to run two programs with identical test parameters back to back. To link identical programs, link the program to itself. To link another program to the one previously selected, select option 2.
4 Operating the 8000 Following is a description of the Select Start Mode screen options: Table 11 Select Start Mode Options (Apparatus 1 / 2) Option Function 1 Start Now Select option 1, Start Now. The DISSO Options screen displays. See “DISSO Options Screen” on page 69. 2 Delayed Start Select option 2, Delayed Start. The DISSO Options screen displays. See “DISSO Options Screen” on page 69. 3 Remote Start This option is designed for use with the 8000 coupled with non-Agilent dissolution apparatus.
Operating the 8000 4 DISSO Options Screen ***DISSO OPTIONS*** 1 LOAD & RUN DISSO 2 RUN WO/ LOADING 3 RUN 8000 ONLY 4 SET DISSO TIME: 01/01/12 08:09:51 ELAPSE: 000:00:00 Following is a description of the DISSO Options screen options: Table 12 Disso Options (Apparatus 1 / 2) Option Start Now Function 1 Load & Run DISSO Use this option to upload all saved parameters to the dissolution apparatus and start the program. Select option 1, Load & Run DISSO.
4 Operating the 8000 Table 12 Disso Options (Apparatus 1 / 2) Option Start Now Function Delayed Start Function 3 Run 8000 Only Select option 3, Run 8000 Only, to immediately start the 8000 only. The 8000 executes the program currently selected. Enter the start time in 24-hour format and press ENTER. Enter the start date in the appropriate format and press ENTER. The DISSO Options screen displays.
Operating the 8000 4 Table 13 Select Disso Program Start Mode Options (Apparatus 1 / 2) Option Start Now Function Delayed Start Function 1 Instant Select option 1, Instant, to start the dissolution apparatus instantly. To stop the dissolution apparatus, press ESC. A confirmation screen displays. Press Y to stop the program. Press N to continue running the program. Select option 1, Instant, to set the desired time to start the dissolution apparatus.
4 Operating the 8000 Operating the 8000 with Apparatus 3 / 7 8000 Keypad Options The keypad on the 8000 is similar to a personal computer keyboard. For example, it has a SPACE key, an ENTER key, and a SHIFT key. These keys function exactly like their counterparts on a personal computer or typewriter keyboard. See Figure 15, “8000 Keypad,” below. A discussion about each of the main function keys follows.
Operating the 8000 4 The front panel options include the following: Table 14 Front Panel Options (Apparatus 3 / 7) Option Function ESC Press ESC to stop execution of a running program or calibration routine. Press ESC to move back one level to the previous menu or selection when moving through the multi-level menu. Be very careful when using ESC while a program is running.
4 Operating the 8000 Table 14 Front Panel Options (Apparatus 3 / 7) Option Function CLEAN SYTEM Use this option to keep the valves, needles, and fluid lines clean. See “Clean System with Peristaltic Pump” on page 106 or “Clean System Function with Syringe Pump” on page 111. LOCK KBRD Press LOCK KBRD to lock the keypad. Press LOCK KBRD again to unlock the keypad. 8000 with Apparatus 3 / 7 - Menu Options Main Menu From the Ready screen, press MENU. The Main Menu displays.
Operating the 8000 4 Following is a description of the Main Menu screen options: Table 15 Main Menu Options (Apparatus 3 / 7) Option Function 1 Set Clock/Alarms Use this option to set the clock and alarms. If your system configuration includes the syringe pump, the start output delay option also displays on this screen. See “Main Menu Option 1 - Set Clock/Alarms” on page 77. 2 Set APP 3/7 Use this option to set the Apparatus 3 / 7 parameters. See “Main Menu Option 2 - Set DISSO” on page 53.
4 Operating the 8000 Table 15 Main Menu Options (Apparatus 3 / 7) Option Function 4 Manual Prime Vol. (syringe pump) If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system. Select option 4, Manual Prime Vol. Enter a value for the priming volume and press ENTER. The Main Menu displays.
Operating the 8000 4 Main Menu Option 1 - Set Clock/Alarms From the Main Menu, select option 1, Set Clock/Alarms. The Set Clock and Alarms screen displays. ***SET CLOCK AND ALARMS*** 1 SET CLOCK 2 TIMER ALARM 3 ROW COMPL ALARM 4 CALIB. COMPL ALARM 5 PRG COMPL ALARM 6 START OUTPUT DELAY Following is a description of the Set Clock and Alarms screen options: Table 16 Set Clock and Alarms Options Option Function 1 Set Clock Select option 1, Set Clock.
4 Operating the 8000 Table 16 Set Clock and Alarms Options Option Function 4 Calib. Compl Alarm Use this option to set an alarm to sound when autocalibration is complete and all lines are purged. Select option 4, Calib. Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays. Press any key to silence the alarm after it sounds. Note: This option is only available if your system configuration includes the peristaltic pump.
Operating the 8000 4 Main Menu Option 2 - Set APP 3/7 From the Main Menu, select option 2, Set APP 3/7. Enter a program number and press ENTER. The Set APP 3/7 Parameters screen displays.
4 Operating the 8000 Table 17 Set App 3/7 Parameters Options (Apparatus 3 / 7) Option Function 5 Com Port ID Select option 5, Com Port ID, to change the communication port identification number. Your system was shipped with the communication port identification number set to 01. You can set the identification number to any number between 1 and 99. The identification number is used when multiple units are connected in series so that the controlling or master 8000 can start individual units.
Operating the 8000 4 From the Main Menu, select option 3, Set Syringe Pump. Select option 2 to disable the syringe pump or select option 1 to enable the syringe pump. Once enabled, the Set Syringe Pump Parameters screen displays. ***SET SYRINGE PUMP PARAMETERS*** 1 SYRINGE SIZE 2 PLUNGER SPEED 3 PRIME LOSS VOLUME 4 ASPIRT.
4 Operating the 8000 Table 18 Set Syringe Pump Parameters Options (Apparatus 3 / 7) Option Function 4 Aspirt. Dwell Time Use this option to configure the aspiration dwell time. Aspiration dwell time is the time the syringe plunger pauses at the bottom of a stroke to allow the medium being drawn to overcome the vacuum generated by the drawn plunger. Select option 4, Aspirt. Dwell Time. Enter an aspiration dwell time between 1 and 10 seconds and press ENTER. The recommended time is 10 seconds.
Operating the 8000 4 The following options are commands communicated from the 8000 to the Apparatus 3 / Apparatus 7: Table 19 App 3/7 Control/Monitor Options Option Function 1 Dip Select option 1, Dip, to begin dipping. 2 Stop Select option 2, Stop, to stop dipping and raise the cannulas. 3 Go To Row Select option 3, Go To Row, to move the drive unit to a specific row. Enter a row number and press ENTER. The Apparatus 3 / 7 drive unit moves to the indicated row.
4 Operating the 8000 Following is a description of the Manual Operations screen options: Table 20 Manual Operations (Apparatus 3 / 7) Option Function 1 Lift Valves Select option 1, Lift Valves, to raise the valve needles if lowered. 2 Lower Valves Select option 2, Lower Valves, to lower the valve needles if raised. 3 Open/Close Valves Select option 3, Open/Close Valves, to toggle the valves open and closed. 4 Goto Rows Select option 4, Goto Rows, to move the 8000 dispensing arm to a specific row.
Operating the 8000 4 Following is a description of the Operate Syringe/Filter Changer screen options: Table 21 Operate Syringe/Filter Changer Options Option Function 1 Valve to Input The syringe pump contains a valve to control the direction of the flow of medium. Use this option to move the valve to the input position to manually input or fill the syringe with medium. Select option 1, Valve to Input, to move the valve to the input position. The valve emits a loud click when it moves.
4 Operating the 8000 Table 21 Operate Syringe/Filter Changer Options Option Function 5 Filter Changer Use this option to manually operate the filter changer. Select option 5, Filter Changer. The Filter Change Manual Control screen displays. To open the filter clamp, select option 1, Open Clamp. To close the filter clamp, select option 2, Close Clamp. To change the filters, select option 3, Change Filters. Enter the number of filters to change and press ENTER.
Operating the 8000 4 The following Program Variables screen displays when your system configuration includes the peristaltic pump: ***PROGRAM VARIABLES*** 4 HEADERS 1 SET APP 3/7 PARAMETERS 5 PRIME TIME 2 SAMPLE TIME POINTS 6 PURGE TIME 3 SAMPLE VOLUME 7 R-M The following Program Variables screen displays when your system configuration includes the syringe pump with or without the filter changer: NOT E ***PROGRAM VARIABLES*** 4 HEADERS 1 SET APP 3/7 PARAMETERS 5 PRIME VOLUME 2 SAMPLE TIME PO
4 Operating the 8000 Following is a description of the Program Variables screen options: Table 22 Program Variables Options (Apparatus 3 / 7) Option Function 1 Set APP 3/7 Parameters Use this option to set the Apparatus 3 / 7 parameters. Select option 1, Set APP 3/7 Parameters. The Set APP 3/7 Parameters screen displays. See “Main Menu Option 2 Set DISSO” on page 53 for a description of the Set APP 3/7 Parameters screen options. 2 Sample Time Points Use this option to enter the sample time points.
Operating the 8000 4 Table 22 Program Variables Options (Apparatus 3 / 7) Option Function 6 Purge Time (peristaltic pump) If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn. Select option 6, Purge Time. Enter a value for the purge time from 1 to 99 seconds and press ENTER. The Program Variables screen displays.
4 Operating the 8000 Table 22 Program Variables Options (Apparatus 3 / 7) Option Function 7 Filter Chg (syringe pump and filter changer) If your system configuration includes the syringe pump and filter changer, select option 7, Filter Chg. Enter the desired number of samples each filter should process before being discharged and press ENTER. The recommended value is 1. The Program Variables screen displays.
Operating the 8000 4 The following conventions are used with this screen: • Review previously entered sample time points by pressing ENTER to scroll through the entries. • After all desired sample time points have been entered, enter 000:00 for the last sample time point and press ENTER. If the maximum of ten sample time points are entered, you do not have to enter 000:00 for the last sample time point. • To add additional sample time points, press ENTER until a new row time displays.
4 Operating the 8000 TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04 3 To run a single program, select option 1. NOT E If more than ten rows are required to collect samples, linking a program allows you to run two programs with identical test parameters back to back. To link identical programs, link the program to itself. To link another program to the one previously selected, select option 2.
Operating the 8000 4 Following is a description of the Select Start Mode screen options: Table 23 Select Start Mode Options (Apparatus 3 / 7) Option Function 1 Start Now Select option 1, Start Now. The APP 3/7 Options screen displays. 2 Delayed Start Select option 2, Delayed Start. The APP 3/7 Options screen displays. 3 Remote Start This option is designed for use with the 8000 coupled with non-Agilent instruments.
4 Operating the 8000 Table 24 App 3/7 Options Option Start Now Function Delayed Start Function 1 Load & Run APP 3/7 Select option 1, Load & Run APP 3/7, to upload all saved parameters to the Apparatus 3 / 7 and start the program. Select option 1, Load & Run APP 3/7, to upload all saved parameters to the Apparatus 3 / 7. Enter the start time in 24-hour format and press ENTER. Enter the start date and press ENTER.
Operating the 8000 4 Manual Sample From the Ready screen, press MANUAL SAMPLE to collect a sample from the dissolution apparatus on command without a program. These options are only for manual sampling and have nothing to do with the stored program parameters for purge volume, prime volume, and so on.
4 Operating the 8000 Table 25 Manual Sampling Options Option Function 3 Set Volume Select option 3, Set Volume. Enter a sample volume between 0 and 14 mL and press ENTER. The Manual Sampling screen displays. Note: The volume can contain a decimal point so that fractional milliliters can be collected. Be sure not to enter a volume larger than the collection tubes can contain. 4 Prime Time (peristaltic pump) Select option 4, Prime Time. Enter a value for the priming time and press ENTER.
Operating the 8000 4 Volumetric Calibration (Peristaltic Pump only) NOT E The CAL key on the 8000 can be used only if the peristaltic pump is installed on your system. To calibrate the syringes in the 806 Syringe Pump, see “5 Full Stroke (hidden option)” under “Main Menu Option 3 - Set Syringe Pump” on page 80“. It is critical that sample volumes are accurately and precisely taken.
4 Operating the 8000 5 The 8000 system monitor displays a reminder to ensure the tubing and pump are ready. The Calibrate Sampling Volume screen displays. ***CALIBRATE SAMPLING VOLUME*** CHECK PUMP & TUBING. IF AUTO CALIBRATION INSTALL CALIB FIXTURE BEFORE STARTING! 1 MANUAL 2 AUTOMATIC 6 Select option 2, Automatic. The dispensing arm moves over the autocalibration block and primes the lines according to the manual prime settings.
Operating the 8000 4 Manual Calibration CAUTION Do not use any acidic media to calibrate your 8000. Be sure to rinse and dry the autocalibration block tips after completion. NOT E We suggest calibrating with tap water. Deionized water does not work well with the autocalibration block which utilizes conductivity testing. To perform the calibration yourself, complete the following steps: 1 Remove the sample tray.
4 Operating the 8000 15 Upon completion of the priming cycle, the following screen displays: PRESS TO START PRESS AGAIN WHEN 10 ML IS REACHED MANUAL CALIBRATION OF VALVE # 1 TIME: 01/01/12 21:56:40 ELAPSE: 000:00:00 16 Press OPEN VALVES. The first calibration tube begins filling. 17 Observe the first calibration tube and press OPEN VALVES again when 10 mL is reached. 18 Repeat Steps 17 and 18 for the remaining calibration tubes.
Operating the 8000 4 Checking the Calibration - Volume Accuracy Check the calibration by running a short sampling procedure and dispensing the requested volume into calibrated test tubes: 1 Place an appropriate number of 10 mL calibration tubes in row 9 of the 8000 sample tray. 2 Ensure the sample cannulas of the dissolution apparatus are sufficiently submerged in water. 3 From the Ready screen, press MANUAL SAMPLE.
4 Operating the 8000 Daisy Chaining A series of dissolution apparatus and 8000s can be started at the same time using one 8000 as the master unit. To set up a daisy chain, complete the following steps: 1 Connect the master 8000 START OUTPUT jack to the START INPUT jack of its dedicated dissolution apparatus using a four-pin cable. Connect the START OUTPUT jack of the first dissolution apparatus to the START INPUT jack of a second 8000.
Operating the 8000 4 Each unit starts running the program you entered. NOT E Ensure no start output delays have been set on any of the 8000s. Depending on your configuration, see “3 Start Output Delay (peristaltic pump)” under “8000 Menu Options - Apparatus 1 / 2” on page 48 or “6 Start Output Delay” under “Main Menu Option 1 - Set Clock/Alarms” on page 51.
4 Operating the 8000 Following is a description of the Print Selections screen options: Table 27 Print Selections Options Option Function 1 Batch Information Select option 1, Batch Information, to immediately print the previously entered batch information, if any, for the selected program. This printed data is the header information you can enter via option 4, Headers, on the Program Variables screen.
Agilent 8000 Dissolution Sampling Station Operator’s Manual 5 Maintenance and Troubleshooting Preventive Maintenance 106 Report Center Impact Printer 119 Agilent Technologies 105
5 Maintenance and Troubleshooting Preventive Maintenance WA RNING The 8000 and accessories contain electrical circuits, devices, and components operating at dangerous voltages. Contact with these circuits, devices, and components can cause death, serious injury, or painful electric shock. Perform the following maintenance when necessary: Clean System with Peristaltic Pump Use the Clean System function often to keep the valves, needles, and sample lines clean.
Maintenance and Troubleshooting 5 5 On the 8000, press CLEAN SYSTEM. The Cleaning Systems Selections screen displays. ***CLEANING SYSTEMS SELECTIONS*** 1 START CLEAN 2 ENABLE AUTO CLEAN TIME: 01/01/12 08:28:46 ELAPSE: 000:00:00 To start the cleaning process immediately, see “Start Immediately” on page 108. To program the auto clean function, see “Programmed Clean” on page 108. Apparatus 3 / 7 1 Place the appropriate number of outer media tubes in Row 1 of the Apparatus 3 / 7.
5 Maintenance and Troubleshooting Start Immediately From the Cleaning Systems Selections screen, select option 1, Start Clean. The 8000 immediately starts to clean the system by priming the lines. Once the system completes the priming, the needle manifold jogs up and down while opening and closing the valves. This motion rinses the needles in the 8000 rinse chamber. Then the system purges the cleaning solution from the sample lines.
Maintenance and Troubleshooting 5 Auto Clean. ***CLEANING SYSTEMS SELECTIONS*** 1 START CLEAN 2 PROGRAM AUTO CLEAN 3 DISABLE AUTO CLEAN TIME: 01/01/12 NOT E ELAPSE: 000:00:00 Select option 3, Disable Auto Clean, to escape the automatic clean function and return to the original Cleaning Systems Selections screen. Select option 1, Start Clean, from either screen to escape the automatic clean function and immediately start the cleaning process. 3 Select option 2, Program Auto Clean.
5 Maintenance and Troubleshooting 4 Select each option and enter the appropriate parameters as necessary based on tubing length, tubing diameter, and system dead volumes. Table 28 Program Auto Clean Variables Option Function 1 Clean Frequency Select option 1, Clean Frequency. Enter an interval of up to 99 minutes and press ENTER. The cleaning begins at this time. Note: Ensure the clean frequency is set earlier than the first sample time point. 2 Drawing Media Time Select option 2, Drawing Media Time.
Maintenance and Troubleshooting 5 • the peristaltic pump reverses and the cleaning solution is pulled back through the sample lines • the pump reverses again, the valves open and the cleaning solution is expelled into the rinse chamber • the 8000 activates the valves several times to clean the valves and needles. Clean System Function with Syringe Pump Apparatus 1 / 2 1 Place the rinse tray fitted with cleaning solution, usually water, on the vessel table of the dissolution apparatus.
5 Maintenance and Troubleshooting 9 Upon completion of the cleaning process, the 8000 dispensing arm and the Apparatus 3 / 7 drive unit return to the home position. Syringe and Seal Replacement NOT E If the syringe seal eventually develops a leak, retighten the syringe by hand (18 - 21 oz-in, 1.3 - 1.5 Kg-cm). If the seal eventually obstructs the media path, new seals are available for purchase (P/N K1002-00438). 1 Disconnect all sample tubes from the pump.
Maintenance and Troubleshooting 5 12 Install the new seal into the syringe port, making sure that it lays flat in the bottom of the port and that the media path is not obstructed. 13 Install the syringe into the valve. Tighten by hand (18 - 21 oz-in, 1.3 - 1.5 Kg-cm). 14 Extend syringe plunger so that it is aligned with the plunger pin on the pump. 15 Carefully install the syringe and valve assembly onto the valve and plunger pins. 16 Replace the syringe valve retainer block and tighten the screws.
5 Maintenance and Troubleshooting 5 Enter 100 and press ENTER. The syringes fill completely with cleaning solution. 6 Allow the solution to remain in the syringes for ten minutes. 7 Select option 4, Syringe Dispense. The cleaning solution expels from the syringes. 8 Replace the cleaning solution in the rinse tray with ultrapure water. 9 Repeat Steps 3 - 7 a minimum of ten times to flush the syringes thoroughly. 10 When the cleaning procedure is complete, press ESC until the Ready screen displays.
Maintenance and Troubleshooting 5 System Cleaning Procedure with Syringe Pump and Filter Changer (optional) To clean the 8000, syringe pump, and filter changer system, use the appropriate cleaning solution based on the product being tested. Ensure the sampling cannulas are submerged into the cleaning solution. Complete the following steps to set up the cleaning procedure: NOT E Ensure the RM option is disabled by pressing MENU > 0 > R. 1 From the Ready screen, press PROG to program the 8000.
5 Maintenance and Troubleshooting 6 Enter the following parameters: Table 30 Program Variables Selection 2 Sample Time Points Response Row 0: Enter 000:05 and press ENTER. Row 1: Enter 000:10 and press ENTER. Row 2: Enter 000:15 and press ENTER. Row 3: Enter 000:00 and press ENTER. 3 Sample Volume Enter a sample volume based on the size vial used in the 8000 sample tray and press ENTER. Ensure Rows 0 - 2 in the sample tray contain empty vials.
Maintenance and Troubleshooting 5 6 Repeat Steps 4 and 5 a minimum of four times to completely expel the cleaning solution from the needles. 7 Select option 3, Open/Close Valves, to close the valves. 8 Select option 4, Goto Rows. 9 Press H to move the dispensing arm to the home position. 10 Press ESC until the Ready screen displays. Cleaning the Filter Changer Drip Tray 1 Slide up and remove the filter catch basin from the screw mount on the front of the filter changer.
5 Maintenance and Troubleshooting 9 Press ESC to return to the Manual Operations screen. 10 Select option 5, Turn On R-M Pump. 11 Allow the pump to run until the source beaker is empty. 12 Select option 5, Turn Off R-M Pump. 13 Repeat Steps 2 - 12 using ultrapure water in place of the warm water. 14 Select option 5, Turn On R-M Pump. 15 Purge the system by removing the source line from the ultrapure water and allowing the pump to run until water no longer flows through the return line.
Maintenance and Troubleshooting 5 Report Center Impact Printer The following is helpful information for using your impact printer. Installing the Cartridge Ribbon If the printer is used infrequently, the print impression sometimes becomes weak because the ribbon dries out. If the printed material is difficult to read and you suspect this is the cause of the problem, advance to a properly inked portion of the ribbon by pressing the printer toggle switch into the Paper feed position.
5 Maintenance and Troubleshooting If you get ribbon ink on the printer’s plastic cover, remove it immediately. Once dried, it is difficult to remove. Replacing a Paper Roll 1 Toggle the printer off line by pressing the printer toggle switch to the OnLine / Off Line position. When the printer is off line, the Ready LED does not illuminate. 2 Grasp the paper roll cover firmly by the grooves on the side and the front edge. Pull outward to remove the cover.
Maintenance and Troubleshooting 5 13 Replace the paper roll cover. If the cover is difficult to remove or replace, the left and right edges can be trimmed or shaved with a utility knife allowing the cover to slide easier. 14 Toggle the printer online by pressing the printer toggle switch to the OnLine / Off Line position. The Ready LED illuminates.
5 Maintenance and Troubleshooting 5 Press the printer toggle switch to the OnLine / Off Line position to stop the printing operation. 6 The printer is ready to resume normal operation. Printer Configuration NOT E The printer configuration is set by the factory. This procedure should be performed only if the printer displays erroneous characters. Contact the Dissolution Systems Service Department for assistance, if necessary. 1 Turn off the 8000.
Maintenance and Troubleshooting 5 *** SETUP MENU*** CONFIGURE CUSTOM [NEXT/OK] [NEXT/OK] Press NEXT to avoid configuration. Press OK to enter custom mode. ***CUSTOM MENU*** PRINT CUSTOM SETUP [NEXT/OK] Press NEXT. AUTO SEQ = NO [NEXT/OK] Press OK. ZERO = Ø [NEXT/OK] Press OK. POUND SIGN = # _(UNDERSCORE) ONLINE/OFFLINE=YES [NEXT/OK] [NEXT/OK] [NEXT/OK] Press OK. Press OK. Press OK. EXT CH SET = NO [NEXT/OK] Press OK. PRINT READY = YES [NEXT/OK] Press NEXT.
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Index Numerics F 8000 clamp removal, 21 unpacking, 21 filter changer change filters, 86 filters, 40 operate, 59, 84 power connections, 35 final spin length, 53 full flow filters, 34 A aspirt.
Index R ready screen, 35 removing paper roll, 120 replacement media (rm) installing external, 39 installing internal, 38 replacement media (rm) option, 96 report center printer operation, 103 row compl alarm, 51, 77 rs232, 24 run 8000 only, 94 run wo/ loading, 94 syringe dispense, 60 syringe pump enabling, 42 fill syringe, 85 main menu, 48 operate, 59, 84 power connections, 35 set syringe size, 55, 81 setting, 55, 80 syringe dispense, 85 syringe parameters, 81 unpacking, 26, 29 syringe to fill, 60 S T
