User`s guide

100 User’s Guide
6. Leave the Amount Values set to "1". For details on
how these values are used, see Method
Development.
7. Click on the Calibration checkbox, then enter a "1"
for Calibration Level. Since this method is currently
uncalibrated, it is unnecessary to select any of the
boxes dealing with calibrations or replicates.
However, if you are unsure of the method contents,
click the Clear all calibration box before starting.
8. When you have completed the dialog, click Start.
When the analysis is complete, the chromatogram will
be integrated, and the areas for the peaks identified
as calibration compounds will be entered into the
method. The calibration curves will be generated
using these areas. At this point, the method is
calibrated for a single level, and it can now be used to
run and analyze samples with the calibration
compounds in unknown amounts.
Create and Run a Sample Sequence
If you are using an autosampler to inject samples, you must
define the samples to be injected and how they are to be
acquired and analyzed. This is done using a sample
Sequence. A sample Sequence can be used to acquire both
calibration and unknown samples. It can also be used to
automatically re-analyze stored data files. Details on creating
and using a Sequence are located in the Sequence section. In
this part of the Tutorial you will create and use a simple
sequence to acquire a calibration sample and two or three
unknown samples.
1. To create a new sequence, click the
File>Sequence>Sequence Wizard command. A
Sequence Wizard dialog box will appear.