Specifications

43
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•
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DETECTORS
DAD/MWD Flow Cell Selection
Typical Column
Length (cm)
Typical Peak
Width
Recommended Flow Cell
< = 5 0.025
500 nl
Flow Cell
High Pressure
Flow Cell
10 0.05
Semimicro
Flow Cell
20 0.1
Standard
Flow Cell
> = 40 0.2
Typical Flow Rate 0.05 - 0.2 ml/min 0.2 - 0.4 ml/min 0.4-0.8 ml/min 1-2 ml/min 0.05 - 5ml/min
Internal Column Diameter 0.3 - 1 mm 2.1 3.0 mm 4.6 mm
Step 1: Remove the capillaries.
Step 2: Take out the cell.
Step 3: Remove the cell screw and gasket.
Flow Cell Maintenance Tips
●
Perform the intensity test and detector
cell test. These built-in tests will help you
verify the performance of your detector,
and determine when maintenance is
necessary.
●
Clean or replace the fl
ow cell
windows as needed.
●
Perform a wavelength calibration to
ensure wavelength accuracy
.
●
Clean the leak sensors and check
their function.
●
Inspect the positioning of the waste tube.
●
Exchange the lamp.
●
Perform a dark current and filter test.
Agilent offers eight flow cells for use with
the diode array and multiple wavelength
detectors. Use the chart below to select
t
he appropriate one for your application,
keeping the following guidelines in mind:
Choosing the Optimal Flow Cell
If more than one selection is appropriate,
choose the
larger flow cell to get the best
detection limit.
For columns capable of providing narrower
p
eak widths (1.8 µm particle size), the
smaller flow cell may be more appropriate.
Flow cells with longer path lengths provide
better signal detection. However, a longer
path length also increases the cell volume,
which causes more peak dispersion.
For information on selecting prep cells,
please refer to our technical note
G
1315-90102 under Library Information
at www.agilent.com/chem