Specifications
37
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DETECTORS
Disassembling and cleaning the cell
H
ow to remove the cell:
1
. Remove both capillaries from the flow cell.
2. Unscrew the cell screws and remove the flow cell.
How to disassemble the cell windows:
1. Open the cell with a 4 mm hexagonal key.
2. Use tweezers to remove the conical springs and gaskets.
3. Remove the cell windows with scotch tape.
Important:
Do not remove the flow cell windows with tweezers if
you plan to reuse them. The tweezers will scratch the
windows’ fragile surface.
4. Use ethanol to clean the cell body.
5. Use a cotton swab and ethanol to clean the cell windows.
Note: if you work with proteins, you may want to try a
contact lens cleaner. Or, simply replace the windows.
6. Make sure that no deposits are on the window or cell body.
Reassembling the cell windows and cell
1
. Insert Gasket #2.
N
ote:
T
he hole of gasket #2 is larger
t
han the hole of gasket #1.
2. Install the cell window.
3. Insert Gasket #1.
4. Insert the SST ring.
5. Install the conical springs.
Note: Install the conical springs
with the conical side toward the cell window. This will ensure
correct placement of the conical springs, which is critical
to proper tightness.
6. Carefully tighten the cell screw.
The cell repair kit includes the required cell windows
(pack of 2) and gaskets.
Installing the Detector Cell
Before you return the flow cell to the instrument module …
1. Connect the capillaries.
2. Switch on the pump and check the cell for leaks.
Once you are convinced the cell is leak free …
3. Install the flow cell into the instrument.
4. Screw down the cell.
There are two designs of the Standard Flow Cell. The cell
screw of the new design, G1314-60086, is painted black.
Tips & Tools
A vital parameter of the VWD is backpressure on the cell. Make
certain that you use the appropriate capillary from the detector
flow cell to the waste accessory. Part number: 5062-8535
We recommend the following tests after cell installation:
Zero-order calibration
Wavelength calibration
Because the flow cell is part of the optical system and has
now been moved since these tests were last performed, we
recommend running them again before your next analysis.