Specifications

31
www.agilent.com/chem
•
800 227 9770
PUMP MAINTENANCE
Solvent Filtration
Unfiltered solvents or solvents contaminated with microbial
g
rowth – especially aqueous buffer solutions – present a common
problem in HPLC. Aqueous media encourage microbial growth
that can clog the solvent inlet filter and the HPLC; particulates in
unfiltered solvents can have the same affect. Both microbial
g
rowth and particulates can also impact the performance of the
pump. For instance, blocked solvent inlet filters can cause
erroneous mobile phase composition, which will impact peak
elution time. In addition, if the pump keeps working while the
filters are blocked, the pump could draw in air instead of solvent.
This will result in periodic baseline disturbances.
●
Use sterile solvent bottles, if possible.
●
Filter solvents through sterile filters (< 1 µm).
●
Replace solvents every two days.
●
Avoid exposure to direct sunlight, or use brown glass bottles.
Solvent Filter/Degasser
Description Unit Part No. Price ($)
HPLC Solvent filter/degasser assembly 3150-0577 469
Regenerated Cellulose Filter Membranes
47 mm, pore size 0.45 µm
100/pk 3150-0576 164
Nylon Filter Membranes 47 mm,
pore size 0.45 µm
100/pk 9301-0895 152
PTFE Filter Membranes 47 mm,
pore size 0.45
µ
m
10/pk 3150-0509 42
Glass funnel, 250 ml, for 3150-0577 5188-2743 127
PTFE Coated sieve, for 3150-0577 5188-2744 153
PTFE seal, for 3150-0577 5188-2745 25
Funnel base, glass, for 3150-0577 5188-2746 180
An added benefit of filtering solvents is
that they are degassed at the same time.
This is particularly beneficial if you do not
have an on-line degasser in your system.
The benefits of solvent filtration:
●
Degasses eluents as particulates
are removed.
●
Prevents the formation of spurious
peaks within the detector due to solvent
outgassing at the low-pressure end of
the chromatograph.
To avoid these problems we recommend these precautions:
3150-0577
●
Increases solvent inlet lifetime.
●
Eliminates pump downtime caused by air
locks and particulates in check valves.
●
Decreases piston wear, while increasing
column life.