Operate your lab at peak performance Troubleshoot difficult problems Select the right Agilent supplies Maintaining Your Agilent 1100 Series HPLC System Maximize efficiency. Minimize your downtime. Get the results you need.
We’re committed to bringing you the world’s best HPLC systems. Plus the critical information you need to keep them running properly. As an Agilent customer, you have complete access to over 40 years of industry knowledge and experience. With a wide range of technical know-how and a never-ending supply of ideas, our goal is to help you solve your most challenging application problems. That’s why we created Maintaining Your Agilent 1100 Series HPLC System.
Manual Injection Valves Meticulously engineered to ensure trouble-free operation. You can count on Agilent injection valves, just like you depend on your HPLC System. That’s because our valves are built to Agilent’s industry-leading standards to ensure consistent results and optimum performance from your analytical instruments. In this section, you’ll find the latest in LC injection valve technology, along with part numbers and important maintenance information.
MANUAL INJECTION VALVES Agilent injection valves from Rheodyne are backed by 40 years of innovation and experience. They feature … ● ● Patented Make-Before-Break (MBB) technology that provides uninterrupted flow when switching between LOAD and INJECT positions. Additionally, MBB greatly reduces transient pressure shocks, which benefits flow-sensitive detectors, and extends column life.
MANUAL INJECTION VALVES Series 3725i-038 and 3725i Preparative Injection Valves The series 3725i-038 (stainless steel) and 3725i (PEEK) are the most suitable manual valves for large sample volumes, high flow rates, and preparative columns sized 1.0-10 cm in diameter. ● ● ● ● ● ● Versatile ports accommodate 1/8″ (3.2 mm) and 1/16″ (1.6 mm) OD tubing. (Note: 1/16″ OD tubing requires an adapter, PN 5067-1503.) ● High reproducibility for both partialfilling and complete-filling methods.
MANUAL INJECTION VALVES Maintenance and Replacement Parts for Manual Injection Valves Description Part No.
MANUAL INJECTION VALVES Sample Loops Stainless steel loop ends are square cut and burr-free for a flush connection to the valve. They are also supplied with unswaged fittings. Flexible PEEK loop ends feature a clean, straight cut for low deadvolume connection. Both types of sample loops are factory-cut and finished to the highest quality. Stainless Steel Sample Loops Remember … size designations for loops are nominal. The actual volumes can differ from their theoretical designations because of the ±0.
MANUAL INJECTION VALVES PEEK Sample Loops for 9725 Injectors Description PEEK Sample Loops PEEK is inert to almost all organic solvents and is biocompatible. Like metal loops, the actual volumes of PEEK loops can differ from their nominal designations because of the ±0.05 mm (0.002″) tolerance of the tubing bore. Generally, accuracy rates are about ±14% for large PEEK loops (0.8 mm, 0.030″), ±21% for intermediate loops (0.5 mm, 0.020″), and ±65% for small loops (0.2 mm, 0.007″).
Autosampler Maintenance Ensuring peak performance and increasing your lab’s uptime.
AUTOSAMPLER MAINTENANCE Injection Valve Maintenance Rotor seal replacement is the most common injection valve maintenance procedure. Indications include rotor seal blockages and cross-port leaks. You should suspect a cross-port leak if you notice a drop of mobile phase coming out of the needle when it rises to draw sample from a vial. Or, if you experience poor injection volume precision. Cross-port leaks can also be found by performing a pressure test.
AUTOSAMPLER MAINTENANCE Injection Valve Maintenance Used In (instrument) Description Part No. Price ($) G1313A, G1329A, G1367A 2 Pos/6 Port Injection Valve G1377A 2 Position/6 Port µ Injection Valve 0101-1050 1453 G2258A 10 Port, Dual Loop Valve 0101-1385 1589 G2260A 2 Position/6 Port MBB Injection Valve 0101-1267 1565 0101-0921 1102 RheBuild Kit Part No. 0101-1257 0101-1257 0101-1268 Price ($) 283 283 Rotor Seal Part No.
AUTOSAMPLER MAINTENANCE 3. Move the needle arm to the lowest point using the “Needle Down“ function. Install the new needle. 4. Situate the needle approximately 2 mm above the seat using the “Needle Up” function. Ensure the needle is aligned with the seat. Align the needle above the needle seat. Reconnect the sample loop. Exchanging the Needle Seat (See manual for well-plate Autosampler) Before beginning this procedure: • Select “Change Needle” under “Maintenance Function.
AUTOSAMPLER MAINTENANCE Agilent Autosampler Needle Assembly Description G1313A, G1327A, G1329A Needle assembly, standard autosampler Part No. G1313-87201 Price ($) 56 Compatible with Needle Seat Part No. Price ($) Standard needle seat 0.17 mm ID capillary, 2.3 µl G1313-87101 88 Standard needle seat 0.12 mm ID capillary, 1.2 µl G1313-87103 89 G1313A, G1327A, G1329A Needle assembly, for use with PEEK seat G1313-87203 133 Standard needle seat, PEEK 0.17 mm ID capillary, 2.
AUTOSAMPLER MAINTENANCE Autosampler Parts and Supplies Agilent offers a variety of supplies that increase the flexibility of your 1100 System. Trays for 1100 Series Autosamplers Description Used In (instrument) Part No.
AUTOSAMPLER MAINTENANCE G1313/27/29A 1100 Series Autosampler Supplies Description Part No. Price ($) External Vial Tray for 17 vials (disposal position) G1313-60004 232 Disposal Tube for external vial tray G1313-27302 42 Waste tubing, 5 m, 6.5 mm ID, corrugated polypropylene 5062-2463 23 Needle seat PEEK, 0.17 mm ID capillary, for standard autosampler, 2.
AUTOSAMPLER MAINTENANCE G1367A 1100 Series Well Plate Autosampler Description Part No. Price ($) Well plate sampler accessories kit Standard ship kit, includes 100/pk 2 ml screw top vials & caps, 10/pk 0.5 ml 96 well plates, tools, 380 mm 0.17 mm capillary, CAN cable G1367-68705 278 Needle assembly, well plate autosampler (green) G1367-87200 101 Needle assembly, well plate autosampler (new, for SN greater than DE21001534) G1367-87201 190 Seat assembly, 0.17 mm ID, 2.
Fraction Collectors Reliable, rugged and easy to use. Agilent 1100 Series fraction collectors are designed to meet stringent standards for collection capability. So you never have to compromise on recovery and purity. In this section, you’ll find essential facts about our analytical scale (AS) and preparative scale (PS) fraction collectors – including: • Fraction collector maintenance – plus simple repairs you can perform without a service call.
FRACTION COLLECTORS Analytical and Preparative Fraction Collector Maintenance Fraction collectors/spotters are very user friendly, and do not require much maintenance. However, you should perform the following routine procedures: ● Exchange the tubing at least once per year. ● Replace the capillaries. ● Replace the flap septum. Simple Repairs Tubing Replacement There are two types of tubing available to connect the valve with the needle: preparative scale PTFE tubing (0.
FRACTION COLLECTORS Trays, collecting tubes and plates for 1100 Series Fraction Collectors Agilent offers a broad range of trays, glass collecting tubes and well plates. When deciding what’s best for your application, consider the size and number of the fractions you want to collect. Trays and Collecting Tubes Hole Diameter (mm) No. of Tubes Dimensions Tray Part No. 30 40 30 x 100 mm G1364-84503 Price ($) 261 30 x 48 mm Price ($) Tube Part No.
FRACTION COLLECTORS Well plates Description Unit Part No. Price ($) 96 Well plates, 0.5 ml, Polypropylene 120/pk 5042-1385 195 96 Well plates, 0.5 ml, Polypropylene 10/pk 5042-1386 22 96 Deep well plates, 1.
FRACTION COLLECTORS Needle Assembly Description Item Description Part No. 1 Needle assembly preparative scale G1364-87201 80 2 Needle assembly analytical scale (20 mm) G1364-87202 262 3 Needle assembly analytical scale (50 mm) G1367-87200 101 4 Capillary guiding assembly micro scale G1364-87303 479 1 www.agilent.
FRACTION COLLECTORS Maintaining The Micro Fraction Collector/Spotter Agilent offers three types of connecting capillaries – each with different internal diameters. We recommend replacing the capillaries and flap septum every six months – or when contaminated, blocked, worn, or visibly damaged. Before replacing the capillary, be sure to … 1. Place the transport unit of the micro fraction collector/spotter in the “Home” position. 2. Remove all installed trays from the tray base. 3.
FRACTION COLLECTORS G1364D Micro Fraction Collector Description Part No. Price ($) MALDI spotting adapter for G1364D G1364-83205 107 Pushing adapter assembly for G1364D G1364-60021 124 Flap septum, PEEK, for internal tray G1364-27107 45 Fused silica/PEEK capillary, 25 µm, 50 cm G1364-87304 118 Fused silica/PEEK capillary, 50 µm, 50 cm G1364-87305 121 Fused silica/PEEK capillary, 100 µm, 50 cm G1364-87306 104 G1364-83205 Waste tube, PTFE, 20 cm 1.4 mm ID 2.
Now there’s a cleaner, smarter way to maintain your LC/MS rough pump. Introducing … the Agilent Quiet Cover! Nobody likes changing the oil in an LC/MS rough pump. It’s a heavy, dirty job – from lifting the pump out of its pan, to holding the pump steady while the oil drains. But with the Agilent Quiet Cover, there’s no sweating, straining or spilling. Need to check the oil? Just open the Quiet Cover’s door. Need to add oil? Simply lift the Quiet Cover’s top to access the fill cap.
Pump Maintenance Achieve maximum uptime by following our recommended procedures. The Agilent 1100 Series HPLC system has several different pump units to choose from: isocratic, binary, quaternary, capillary and preparative. Additionally, you can access all pump parts by simply removing the front cover.
PUMP MAINTENANCE Purge Valve – Exchanging the PTFE Frit 1100 standard pump modules Purge valves feature a gold seal held in place with a plastic cap. Small leaks can occur here, so you will need to replace the gold seal after the valves have been removed more than once or twice. When replacing the frit, make sure that the cutout side faces up, which provides more surface area on the side facing the solvent flow. 1. Remove capillary and waste tube. 2. Use a 14 mm wrench to unscrew the valve. 3.
PUMP MAINTENANCE Cleaning or Exchanging the Outlet Ball Valve 1. Remove the capillary from the valve. 5. Replace the gold seal and cap. 2. Use a 14 mm wrench to remove the valve. 6. Use a 14 mm wrench to reinstall the valve. You should change the outlet ball valve as part of your regular maintenance routine – or when the valve is leaking internally. Typically, if the pressure ripple is unstable, you should run a leak test to determine if the outlet ball valve needs to be replaced. 3.
PUMP MAINTENANCE Replacing the Active Inlet Valve cartridge (All pumps except the G1361A preparative pump) You should service the active inlet valve when the pressure ripple is unstable and a leak test confirms a problem with the valve due to internal leaking. The active inlet valve contains a cartridge that you can exchange as follows: Description Part No. Price ($) Cartridge for active inlet valve 5062-8562 164 Active Inlet Valve for 1100 G1312-60010 447 1.
PUMP MAINTENANCE Changing the Pump Seals (All pumps except the G1361A preparative pump) Pump seals should be replaced when there are leaks on the bottom of the pump head, when retention times are inconsistent, or when the pressure ripple is unstable. 1. Disassemble the pump head and use a piston to remove the two seals. 2. Clean the pump chambers and seal areas by flushing with solvent and wiping with a lint-free cloth. 3. Insert new seals. 4. Reassemble the pump head. 5.
PUMP MAINTENANCE Seal Wear-In Procedure Recommended after replacing standard seals only (5063-6589) Important: Do not perform this procedure when installing normal phase seals (0905-1420). It will cause severe damage to the seals. Tools ● Solvent bottle or beaker ● Isopropanol ● AIV adapter (0100-1847) ● Restriction capillary (5022-2159) 1. Fill the bottle with 100 ml of isopropanol, and place it in the solvent cabinet.
PUMP MAINTENANCE Pump Head with Seal Wash Kit (Used with standard and capillary pump) The routine use of highly concentrated buffer solutions (100 mM) will reduce the life of the seals and pistons in your 1100 Series pump. But you can counteract the problem with one of our seal wash kits, which flushes the backside of the seal with a wash solvent; water/isopropanol (90/10) is recommended as the wash solvent.
PUMP MAINTENANCE G1361A 1100 Series Prep Pump Description Part No.
PUMP MAINTENANCE Solvent Filtration Unfiltered solvents or solvents contaminated with microbial growth – especially aqueous buffer solutions – present a common problem in HPLC. Aqueous media encourage microbial growth that can clog the solvent inlet filter and the HPLC; particulates in unfiltered solvents can have the same affect. Both microbial growth and particulates can also impact the performance of the pump.
PUMP MAINTENANCE Cleaning or Replacing the Solvent Inlet Filters Clean or replace the solvent inlet filters at least once every 3 months. Stainless steel filters can be sonicated in isopropanol; however, glass filters cannot be sonicated as glass particles may break off or the filters may shatter. To test if a solvent inlet filter is blocked, disconnect the solvent inlet tube at the end furthest from the filter and bottle head assembly.
PUMP MAINTENANCE In-Line Filters Column inlet frit contamination can increase column back pressure and reduce efficiency. Microbore column blockages are a particular problem, due to the small diameter of the inlet frit. To prevent blockages, always use the appropriate filters in your LC system. Agilent offers two types of high pressure in-line filter kits for use with any HPLC system: Universal solvent filter A high-capacity filter with built-in efficiency.
PUMP MAINTENANCE Vacuum Degasser Parts and Supplies G1322A 1100 Series Vacuum Degasser Description Part No.
Detector Maintenance Promoting optimal performance and functionality. To maintain your HPLC System’s level of productivity, you need to perform regular maintenance on your variable wavelength, diode array and multiple wavelength detectors. In the pages to come, you’ll discover tips and techniques that will help you keep your detectors operating at maximum efficiency. For example, you’ll learn how to … • Choose the most compatible flow cell for your unique application.
DETECTORS Variable Wavelength Detector It is critical to verify the function of the VWD on a regular basis. There are two built-in tests to help you accomplish this: ● ● The wavelength calibration test. The lamp intensity test. If the lamp intensity has diminished more than is tolerable for your application, you will need to replace the lamp. Additionally, you should perform the following routine maintenance procedures: ● Check the waste outlet. ● Clean the leak sensor. ● Check the photocurrent.
DETECTORS Disassembling and cleaning the cell Reassembling the cell windows and cell How to remove the cell: 1. Insert Gasket #2. Note: The hole of gasket #2 is larger than the hole of gasket #1. 1. Remove both capillaries from the flow cell. 2. Unscrew the cell screws and remove the flow cell. How to disassemble the cell windows: 1. Open the cell with a 4 mm hexagonal key. 2. Use tweezers to remove the conical springs and gaskets. 3. Remove the cell windows with scotch tape.
DETECTORS G1314-60086 Standard Flow Cell Replacement Parts 1 Item Description Unit Part No.
DETECTORS G1314-60080 Standard Flow Cell Replacement Parts Item Description Unit Part No.
DETECTORS G1314-60081 Micro Flow Cell Replacement Parts Item Description Unit Part No.
DETECTORS Diode Array Detector (DAD), Multiple Wavelength Detector (MWD) DAD Routine Maintenance Procedures: Perform the intensity test and detector cell test. These built-in tests will help you verify the performance of your diode array, and determine when maintenance is necessary. ● ● ● Clean or replace the flow cells as needed. Perform a wavelength calibration to ensure wavelength accuracy. Clean the leak sensors and check their function. ● Inspect the positioning of the waste tube.
DETECTORS Changing the Deuterium Lamp You should replace the deuterium lamp when the baseline noise and/or drift exceed your application limits – or if the lamp does not light. Use the following procedure: Description Part No. Price ($) Deuterium lamp, 1100 DAD/MWD longlife 5181-1530 852 1. If the detector has been in use, turn it off and allow it to cool before proceeding. Deuterium lamp, 1100 DAD/MWD 2140-0590 581 2. Remove the front cover.
DETECTORS If more than one selection is appropriate, choose the larger flow cell to get the best detection limit. Choosing the Optimal Flow Cell Agilent offers eight flow cells for use with the diode array and multiple wavelength detectors. Use the chart below to select the appropriate one for your application, keeping the following guidelines in mind: For columns capable of providing narrower peak widths (1.8 µm particle size), the smaller flow cell may be more appropriate.
DETECTORS G1315-60012/22 Standard Flow Cell and Replacement Parts Item Description Unit Part No.
DETECTORS G1315-60011 Semi-Micro Flow Cell and Replacement Parts Item Description Unit Part No. Price ($) Semi-micro flow cell 6 mm, 5 µl, 120 bar G1315-60011 791 1 Window screw 79883-22402 70 2 Spring washers 5062-8553 13 3 Compression washer 79883-28801 20 4 Window holder 79883-22301 30 5 Quartz window 1000-0488 36 6 Seal BACK 12/pk 79883-68702 105 7 Seal FRONT 12/pk G1315-68710 82 8 Capillary inlet with heat exchanger 0.
DETECTORS G1315-60015 Micro/High Pressure Flow Cell and Replacement Parts Item Description Unit Part No. Price ($) Micro/high pressure flow cell 6 mm, 1.7 µl, 400 bar G1315-60015 1605 1 Window assembly 79883-68703 152 2 Seal ring 79883-27101 43 3 Quartz window 1000-0953 4 Compression washer 79883-28802 33 5 Spring washers 5062-8553 13 6 Window screw 79883-22404 63 7 Capillary inlet with heat exchanger 0.12 mm, 290 mm long G1315-87325 76 8 Capillary oulet 0.
DETECTORS Nano Flow Cells Description Part No.
DETECTORS LC/MS Supplies LC/MSD and LC/MS Trap Supplies 48 Description Part No.
General Supplies Put nearly 40 years of Agilent knowledge to work for your HPLC system. Agilent offers a wide range of meticulously engineered supplies for operating and maintaining your HPLC system – including: • • • • • • LC tubing Connectors Vials and caps Crimpers and decappers Sample preparation solutions Econofilters and more In this section, we’ll review these key components, and help you make the right choices for your lab. You can also visit www.agilent.
GENERAL SUPPLIES Ready-to-Use Econofilter Membrane Syringe Filters Agilent’s industry-standard 25-mm diameter Econofilters are an ideal choice for high sample throughput laboratories. We recommend using them to clarify small-volume HPLC samples that need further analysis – or when particulate matter in the sample solution may cause problems. Features include … ● ● The same quality membranes found in Agilent’s high-performance syringe filters.
GENERAL SUPPLIES LC Tubing and Fittings What is the Function of LC tubing? How can I minimize problems with my LC tubing? LC tubing creates the sample flow path through your LC system – from the autosampler or manual injection valve … through the column … to the detector. The ideal tubing provides an inert surface and leak-free connections, while eliminating dead volumes.
GENERAL SUPPLIES Standard Fittings for 1/16” od Capillaries Description 0350-1402 5062-8541 Unit Part No.
GENERAL SUPPLIES Universal Connecting Capillaries ● ● ● ● ● Made of flexible stainless steel (0.6 mm OD) with 1/16“ fittings for both ends. Pre-swaged fittings are assembled according to Swagelok specifications. Squarely cut, pre-cleaned and ready to use. Use with SS fittings and ferrules (5062-2418) or PEEK fittings (0100-1516). Non-swaged capillaries include fittings, but not assembled. Flexible Stainless Steel Capillaries (without fittings) ID (mm) Length (cm) Color Code Volume (µl) 0.12 10.
GENERAL SUPPLIES Flexible Capillary Connectors From To ID (mm) Pump Autosampler 0.17 60 SS Green Pre-swaged G1312-67305 56 0.25 13 SS Blue Pre-swaged 01090-87308 98 0.25 32 SS Blue Pre-swaged 79835-87638 105 0.17 50 SS Green With fittings G1328-87600 57 Manual Valve 0.17 90 SS Green 1 end pre-swaged G1329-87300 65 Universal 0.25 70 SS Blue 1 end pre-swaged 01018-67305 75 0.25 70 Ta Blue 1 end pre-swaged 01019-67305 528 0.
GENERAL SUPPLIES Capillary and Fitting Kits Description Contents Part No. Capillary/fitting starter kit, 0.17 mm ID Kit includes: Qty 1 - PEEK capillary 0.17 mm id, 1.5m Qty 4 - Stainless steel capillary, 105 x 0.17 mm Qty 4 - Stainless steel capillary, 150 x 0.17 mm Qty 2 - Stainless steel capillary, 200 x 0.17 mm Qty 2 - Stainless steel capillary, 280 x 0.17 mm Qty 1 - Stainless steel capillary, 400 x 0.17 mm Tubing cutter for PEEK capillaries 1/16 in. stainless steel fittings, 10/pk 1/16 in.
GENERAL SUPPLIES PEEK Coated Fused Silica Capillaries for use with 100 µl/min Flow range 56 Item From To Fitting ID (µm) Part No.
GENERAL SUPPLIES Replacement Fittings and Ferrules for Capillary and Nano Flow System Graphic Description Type Part No.
GENERAL SUPPLIES Capillaries for Nano LC System Description Part No.
GENERAL SUPPLIES 6 ml Vials and Caps for 1100 Series Description Unit Part No.
GENERAL SUPPLIES Vial Kits Description Part No.
Services and Support Every Agilent LC and LC/MS system is backed by our nearly 40 years of experience with instrument design, lab operations, business processes, and regulatory requirements. We stand behind our products with … • Immediate technical assistance by phone or online. • Prompt onsite service from Agilent-certified Engineers. • A variety of service options, including maintenance, repair, compliance, and consultation.
SERVICES AND SUPPORT A Total Commitment to Your Lab for One Fixed Cost Agilent service agreements operate under a yearly fixed cost, which includes preventative maintenance, compliance, telephone support, repairs, parts, and labor.
SERVICES AND SUPPORT Agilent Services Features Telephone Service ● ● Software Service ● ● ● Telephone access to skilled Agilent service professionals Available parts option Telephone access to trained technical professionals Enhancements to the original application software purchase Software bulletins that identify discovered defects and recommend workarounds Advantages to You ● ● ● ● ● ● Onsite Instrument Repair For selected Agilent instruments ● ● ● Offsite Instrument Repair For selec
SERVICES AND SUPPORT Agilent Services Operational Qualification/Performance Verification (OQ/PV) Features ● ● ● ● Verification and documentation of an instrument’s ability to meet specified criteria Procedures and documentation that fit the requirements of GLP, ISO 9000, and other regulatory agencies Full automation to increase the qualification scope without excess time requirements Measuring equipment that is traceable to national and international standards.
SERVICES AND SUPPORT Agilent Services Features Network Qualification ● ● ● Network definition and performance testing using established Agilent Design, Installation Qualification, and Operational Qualification protocols Documentation of the definition, integrity, supportability, and health of high-risk network segments. Completed protocols, created using hardware and software network testing tools.
SERVICES AND SUPPORT Education & Training Services Highly trained lab professionals can boost your productivity, minimize errors and reruns, and expand your chromatography capabilities. And that’s why Agilent offers several training options that cover everything from troubleshooting and maintenance to the most advanced operational techniques.
SERVICES AND SUPPORT Classroom Training Agilent’s training courses for liquid chromatography and mass spectrometry help new and experienced lab professionals learn proper and efficient ways to use analytical instruments and software. These ISO-registered courses also target those who want to broaden or sharpen their troubleshooting, maintenance, and system operation skills. Course Title Contact Agilent today for more information about our training and services. Or visit www.agilent.
SERVICES AND SUPPORT Support Tap Agilent’s LC and LC/MS expertise anytime. Just give us a call to discuss hardware, software, application concerns, or basic operation techniques. ● ● Visit Agilent online For a wealth of knowledge, tips, and insights, go to www.agilent.com/chem. You’ll find: ● ● ● Frequently Asked Questions about Agilent instruments and supplies. Interactive Troubleshooter A step-by-step approach to help you solve common problems.
1100 Series LC System Recommended Maintenance Schedule Pumps ITEM TYPICAL SCHEDULE ACTIONS/COMMENTS PTFE frits in purge valve Every month Before re-installation of the purge valve, always check the gold seal and replace if deformed. Solvent inlet filter Every 6 months Never use the system without a filter installed. Piston seals, outlet gold seal, plastic cap, wash seal, gasket wash seal Every 12 months When piston seals are exchanged, check the surface of the piston for scratches and deposits.
www.agilent.com/chem For detailed information about supplies from Agilent, please contact your local Agilent sales representative, or your authorized distributor, or visit us online. Ask about Agilent’s other valuable resources: • Agilent ZORBAX Column Selection Guide for HPLC • Maintaining your Agilent GC and GC/MS Systems • Essential Chromatography Catalog • Online Library and Tech Support The information and pricing in this guide are subject to change without notice. © Agilent Technologies, Inc.
