Technical data

Methods with Acquisition Parameters 3
Setting parameters to acquire a data file in All Ions MS/MS mode
Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide 93
Setting up an experiment with two or three high energy
experiments allows the analysis of a large number of target
compounds that span a wide variety of compound stability.
While this allows selecting fragment ions with higher signal
from an optimized collision energy, it also decreases the time
that is spent on the precursor ion in the low energy channel,
thereby decreasing its signal. In a 2-channel, 3-channel and
4-channel experiment, the amount of time spent on the
collection of the precursor ion intensity is 50%, 33%, and 25%.
However, if a qualification of quantitative results on the
precursor ion in the low energy channel via fragment ion(s) in
the high energy channel is required, then the higher of the lower
limit of quantitation (LLOQ) for the precursor ion and the lower
limit of detection (LLOD) for the fragment ions will be the
actual LLOQ, and it may make sense to conduct a multi
high-channel experiment.
When you are setting up the experiments, you have the first
experiment have the low energy value, and then you increase
the Collision Energy or Fragmentor voltage for each successive
experiment. So, the highest Collision Energy or Fragmentor
voltage is in the last experiment.