Technical data

92 Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide
3 Methods with Acquisition Parameters
Setting parameters to acquire a data file in All Ions MS/MS mode
Since All Ions MS/MS is not an isolation MS/MS experiment,
you set the Mode to MS (Seg) on the Acquisition tab. For the
acquisition rate, you should attempt to get 8 to 10 data points
across the chromatographic peak for each collision energy
channel. For a typical peak width of 10 seconds at the base, the
recommended Rate for a 2-channel experiment (for example, 0
and 20 V) is 2 Hz; for a 3-channel experiment (for example, 0, 20
and 40 V) the recommended Rate is 3 Hz, and for a 4-channel
experiment (e.g. 0, 10, 20, 40 V), the Rate is 4 Hz.
It is highly recommended to use Collision Energy values of 10,
20 and 40 volts for the three high energy experiments because
those energies match the Collision Energy values that have
been used to acquire the accurate mass MS/MS spectra in all of
the MassHunter Personal Compound Databases and Libraries
(PCDLs) that can be used by the All Ions MS/MS algorithm in
the Qualitative Analysis program to select fragment ions.
On a TOF instrument, good starting values for the voltages for
pesticides are shown in Table 9.
Figure 44 Second Experiment has a Collision Energy of 20 V
Table 9 Fragmentor voltages for All Ions MS/MS Experiments
Number of Experiments Expt 1 Expt 2 Expt 3 Expt 4
2-channel 110 V 275 V
3-channel 110 V 180 V 300 V
4-channel 110 V175 V250 V325 V