Technical data

Methods with Acquisition Parameters 3
Setting parameters to acquire a data file in All Ions MS/MS mode
Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide 91
Setting parameters to acquire a data file in All Ions MS/MS
mode
In order to acquire a data file in All Ions MS/MS mode, it is
necessary to set up a method that has a Time Segment with at
least two and a maximum of four different Experiments
containing different Collision Energy values on a Q-TOF or
different Fragmentor voltages on a TOF.
The low energy Experiment provides MS information, and the
high energy Experiments provide MS/MS fragment information.
See the Qualitative Analysis Familiarization Guide for LC/MS
for more information on analyzing a data file that was acquired
in All Ions mode.
If you have a Q-TOF instrument, you set the Collision Energy to
0 in the first Experiment. In the subsequent Experiments
(channels), you set the Collision Energy to a higher value (for
example, 20 V). If you are creating a method for a Q-TOF with
different collision energies, you set the Fragmentor voltage at
the same value in each experiment. It should be set to a low
enough setting that no fragments are generated in the source
but at a high enough setting that you get enough precursor ions
transmitted to the skimmer. This value depends on the
compounds you are analyzing and their stability. You set the
Collision Energy and the Fragmentor voltage on the Source
tab.
Figure 43 First Experiment has a Collision Energy of 0 V