Technical data

Methods with Acquisition Parameters 3
Setup of TOF and Q-TOF reference mass correction (recalibration)
Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide 87
Enabling reference mass correction
You set up and enable reference mass correction in the Ref Mass
tab within the TOF tab or the Q-TOF tab of the Method Editor
window.
If you mark the Enable check box, the system uses reference
masses of the mass reference standard for automatic
recalibration of each acquired spectrum.
You must select the reference masses for each polarity in the
method. Reference masses are not only ion source dependent,
but also polarity dependent. To adjust both the a and T0 terms
requires at least two reference masses, spread across the
application mass range, such that the first reference mass is
<330 m/z and the second is 500 m/z or more above the first.
Using more reference masses improves recalibration results. Up
to 20 masses can be selected.
The reference masses specified for the method will be stored
with the method. These reference masses are also stored in the
file with acquired data as TOF or Q-TOF method parameters.
If you mark the Use Bottle A check box, the TOF or Q-TOF
controls the valve to introduce the internal reference standard
to the reference sprayer via the CDS.
The Ref Nebulizer parameter is only available if the source is
either Dual ESI, Dual AJS ESI or Dual nanoESI.
Figure 41 Reference Masses tab