Technical data

86 Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide
3 Methods with Acquisition Parameters
Setup of TOF and Q-TOF reference mass correction (recalibration)
Setup of TOF and Q-TOF reference mass correction
(recalibration)
You must do mass corrections
during a run in order to attain the
mass accuracy that Agilent
specifies for the TOF and Q-TOF.
Many applications need as small a deviation of accurate mass as
possible. To obtain this accuracy, you recalibrate the mass axis
for every spectrum with measurements of known reference
masses (i.e “lock masses”). You measure the masses of reference
compounds in a reference standard, which can be the Agilent
reference standard or one of your own choosing.
You can introduce the reference standard into the TOF system
in one of three ways:
• A calibrant delivery system (CDS) that automatically
introduces the standard to a reference sprayer (second
sprayer)— Dual ESI (0.2 to 1.5 mL/min flow rates only), Dual
AJS ESI and Dual nano ESI
• An external dispense pump—other ion sources
• Addition to the sample—other ion sources
You can also use known ions in your sample, such as the “y1” in
a tryptic digest, as a supporting reference mass.