Technical data

62 Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide
2 Instrument Preparation
Tune Set Point Modifications for Medium and Large Proteins
Tune Set Point Modifications for Medium and Large Proteins
For medium and large proteins, the charge envelope may extend
beyond 3200 m/z. It is recommended that you acquire your
intact protein data in the Extended Mass Range (1 GHz) mode
on the Agilent 6200 Series TOF and Agilent 6520/6530 Q-TOF as
this allows the extended mass range needed for larger proteins.
If you are acquiring a protein and the charge states are “cut-off”
in one of the other modes, you can switch to this mode with the
extended mass range.
If you are acquiring medium and large protein data, it is also
recommended that the Quad AMU be raised to 300 amu instead
of using the value established by the Auto Tune algorithm. This
provides the Quad with enough power to transmit the high mass
ions through the quad. Failure to increase the Quad AMU will
result in a mass spectrum where the charge envelope appears to
be “cutoff” for the higher charge state ion clusters.
To adjust the Quad AMU, do the following steps:
1 Switch to the Tune Context.
2 Click the Manual Tune tab.
3 Click the Quad Tab.
4 For the Quad AMU, type 300 for the new value.
5 Save the tune value to save the new Quad AMU value. Then
exit the Tune Context.