Technical data

Instrument Preparation 2
Tuning choices
Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide 57
The Standard Tune (TOF) and Quad Tune do subsets of the
Initial Tune (TOF) and Initial Quad Tune actions, and they use
the current tune parameters as starting points rather than
using the factory defaults.
Standard Tune (TOF) takes about 10 to 15 minutes, and Quad
Tune takes about 10 to 15 minutes to complete.
Quick Tune (TOF)
Quick Tune (TOF) automatically adjusts the most commonly
required subset of tunable parameters.
You can per form a Quick Tune with any instrument mode
selected and with any of the following sources installed:
• ESI
• AJS ESI (Agilent Jet Stream ESI)
• Dual ESI
• Dual AJS ESI (Dual Agilent Jet Stream ESI)
• MMI
• APPI
• APCI
Quick Tune takes 3 to 5 minutes because the software is
optimizing only the most significant parameters followed by a
calibration.
During Quick Tune the system goes through the following steps:
a Automatically opens the calibrant valve
b Adjusts the vertical Q (Vert Q) and bottom slit parameters
in the Beam Shaping Optics (optimizes transmission)
c Adjusts the middle mirror of the compound TOF ion
mirror (optimizes resolution)
d Does a mass axis calibration after the final adjustment
e Prints a tune report