Technical data

56 Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide
2 Instrument Preparation
Tuning choices
For Q-TOF instruments, after running an Initial Tune (TOF),
you need to adjust the Collision Cell Gas pressure. You can find
this procedure in the online Help.
During the Initial Quad Tune process, the system goes through
steps a, c, d and e.
Check Tune
The Check Tune report lets you know if the mass calibration
and optimization limits are met with a Pass or Fail.
You can per form a Check Tune with any instrument mode
selected and with any of the following sources installed:
• ESI
• AJS ESI (Agilent Jet Stream ESI)
• Dual ESI
• Dual AJS ESI (Dual Agilent Jet Stream ESI)
• MMI
• APPI
• APCI
Agilent designed the instrument so that it does not need
frequent autotunes. Periodically, you may want to perform
Check Tune and Check Quad Tune to ensure that the
parameters are still optimized. You do not need to perform a
Check Quad Tune as often as a Check Tune (TOF).
Standard Tune
These tools perform many of the same optimization operations
as the Initial Tune tools but use the current settings as starting
values and does a limited set of ion optic ramps.
Table 2 on page 53 shows all of the sources that you can use to
run a Standard Tune. On all instrument models, you can
perform a Standard Tune with a Dual ESI source.