Technical data

52 Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide
2 Instrument Preparation
TOF mass calibration
Before you calibrate the instrument, you have to set the
instrument state to the proper instrument mode, mass range
and fast polarity switching mode. You set these values on the
Instrument State tab.
When you change the mass range or enable/disable fast polarity
switching on the Instrument State tab, the pulser frequency is
changed which results in the DEI pulser warming up or cooling
down. If the calibration is performed too soon, the DEI may still
be heating up or cooling down which can result in drift. See the
online Help for more information on the Instrument State tab.
At startup, you do a full mass calibration, either manually or
automatically. Automatic calibrations take place when you
select Initial TOF Autotune, TOF Tune or Quick TOF Tune. You
set up a manual calibration from the TOF Mass Calibration tab
in the Tune window (Figure 20).
Figure 20 Standard TOF Mass Calibration tab of the Tune window
See Chapter 3, “Methods with
Acquisition Parameters,” starting
on page 71, to learn more about
mass correction using reference
standards.
During sample analysis the system corrects the calibration with
the introduction of a standard containing reference masses, if
you enable the correction through the method.
The calibration equations used are proprietary.