Technical data

50 Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide
2 Instrument Preparation
Column equilibration and conditioning
• With a method in an interactive run
You can save the method with the set points mentioned in the
above paragraph, and then do a run. The run uses the
method stop time. You can also use a post run time within a
sample method to equilibrate the column.
For more information on worklists,
see Chapter 4, “Data Acquisition”.
• With a parameter in a worklist
You can set up a blank run in a worklist to use as your
equilibration run. Or you can set up an equilibration time for
any sample run, where the system waits the specified time
before injecting the sample. For both cases, the data is
stored.
Conditioning
You can condition a column in one of three ways:
• With one of the first two procedures described in the
Equilibration paragraphs.
You enter pump conditions to bring the column to its initial
condition. You can also condition the column by setting a
post-run time in the method.
• With a script in a worklist
The LC conditioning script, SCP_LCCondition, is part of the
Data Acquisition software. When you enter the script into the
worklist, you specify the method that you will use for the run.
If a TOF or Q-TOF is connected to the LC, you can also enter
a parameter that diverts the LC eluent to waste. With this
script, there is no injection and no data storage.