Technical data

48 Agilent 6200 Series TOF and 6500 Series Q-TOF LC/MS System Concepts Guide

2 Instrument Preparation

Column equilibration and conditioning

You can set up to equilibrate or condition a column in different

ways with the Data Acquisition program.

Equilibration Column equilibration eliminates any previously separated

compounds or impurities from the column after runs with

solvent of a single composition. To equilibrate a column before a

sample run, you pass the solvent that you intend to use for the

run through the column for a period of time.

Conditioning Column conditioning returns column characteristics to their

initial state after a gradient run. To condition a column before a

sample run, you pass the solvent of initial composition through

the column for a period of time.

Equilibration

You can equilibrate a column in one of three ways:

Thermostat. Column

Compartment (TCC)

Turn the right or left controller on or off Right-click the device panel and click

Configuration

Set the maximum controller temperatures Right-click the device panel and click Set

Temperature

Enter information for the instrument columns Tools > Analytical Column Setup

Diode Array Det. (DAD) Turn UV or Visible lamp on Right-click the device panel and click UV

Lamp On; Vis Lamp On

Set the date or time for automatic DAD turnon Right-click the device panel and click Control

Calibrate the DAD wavelength Right-click the device panel and click

Calibration

Bring the baseline of the plot to zero Right-click the device panel and click

Balance

Show an intensity plot for the detector Right-click the device panel and click

Intensity plot

Table 1 Tasks to set up the LC modules (continued)

If you have this module: And you want to: How: