Technical data

8 SureSelect
XT2
Target Enrichment System for Illumina
Contents
3 Sample Preparation (100 ng DNA Samples) 37
Step 1. Shear DNA 38
Step 2. Assess quality (optional) 41
Step 3. Repair the ends 43
Step 4. Purify the sample using AMPure XP beads 45
Step 5. Adenylate the 3' end of the DNA fragments 47
Step 6. Ligate the pre-capture indexing adaptor 48
Step 7. Purify the indexed DNA using AMPure XP beads 49
Step 8. Amplify the indexed library 50
Step 9. Purify the amplified library with AMPure XP beads 51
Step 10. Assess quality with the 2100 Bioanalyzer DNA 1000 Assay 52
4 Hybridization 55
Step 1. Pool indexed DNA samples for hybridization 56
Step 2. Hybridize gDNA library pools to the SureSelect Capture Library 57
Step 3. Prepare streptavidin-coated magnetic beads 59
Step 4. Capture the hybridized DNA using streptavidin beads 60
5 Post-Capture Sample Processing for Multiplexed Sequencing 63
Step 1. Amplify the captured libraries 64
Step 2. Purify the amplified captured libraries using
AMPure XP beads 66
Step 3. Assess quality with the 2100 Bioanalyzer High Sensitivity DNA
assay 68
Step 4. Prepare samples for multiplexed sequencing 70
Step 5. Optional: Quantify captured library pools by QPCR 72
Step 6. Optional: Pool captured libraries for sequencing 73
6 Reference 75
Reference Information for Kits with Revised Index Configuration (indexing primers
in white-capped tubes or blue plate) 76
Kit Contents 76
Nucleotide Sequences of SureSelect
XT2
Indexes A01 to H12 79