Technical data

5 Post-Capture Sample Processing for Multiplexed Sequencing
Step 6. Optional: Pool captured libraries for sequencing
74 SureSelect
XT2
Target Enrichment System for Illumina
3 If you store the library before sequencing, add Tween 20 to 0.1% v/v
and store at –20°C short term.
4 Proceed to template denaturation and flow cell preparation. Refer to
the appropriate Illumina protocol.
Specific library pool dilution and processing can vary based on the flow
cell capacity and analysis pipeline versions being used. Refer to the
appropriate Illumina user guide for instructions.
See page 71 for sequencing run setup guidelines for SureSelect
XT2
libraries.
Table 32 Example of capture pool volume calculations for a 20-
µL final sequencing sample
pool containing 10 nM DNA
Component V(f) C(i) C(f) # Volume to use (µL)
Sample 1 20 µL 20 nM 10 nM 6 5.0
Sample 2 20 µL 15 nM 10 nM 6 6.7
Low TE 8.3