Technical data

SureSelect

XT2

Target Enrichment System for Illumina 7

Content

1 Before You Begin 11

Overview of the Workflow 12

Procedural Notes 13

Safety Notes 13

Required Reagents 14

Required Equipment 16

Optional Reagents and Equipment 17

2 Sample Preparation (1 µg DNA Samples) 19

Step 1. Shear DNA 20

Step 2. Assess quality (optional) 23

Step 3. Repair the ends 25

Step 4. Purify the sample using AMPure XP beads 27

Step 5. Adenylate the 3' end of the DNA fragments 29

Step 6. Ligate the pre-capture indexing adaptor 30

Step 7. Purify the indexed DNA using AMPure XP beads 31

Step 8. Amplify the indexed library 32

Step 9. Purify the amplified library with AMPure XP beads 34

Step 10. Assess quality with the 2100 Bioanalyzer DNA 1000 Assay 35