Technical data
Post-Capture Sample Processing for Multiplexed Sequencing 5
Step 3. Assess quality with the 2100 Bioanalyzer High Sensitivity DNA assay
SureSelect
XT2
Target Enrichment System for Illumina 69
If the yield is too low or non-specific peaks are observed in the
electr
opherogram, repeat the PCR with more or fewer cycles. The goal is
to minimize cycles, while you produce enough library for application to
the flow cell.
Figure 6 Analysis of amplified captured DNA using the High Sensitivity DNA Assay.