Technical data

Post-Capture Sample Processing for Multiplexed Sequencing 5
Step 2. Purify the amplified captured libraries using AMPure XP beads
SureSelect
XT2
Target Enrichment System for Illumina 67
16 Put the plate in the magnetic stand and leave for 2 to 3 minutes, until
the solution is clear.
17 Remove the cleared supernatant (approximately 30 µL) to a fresh tube
or plate well. You can discard the beads at this time.
18 Remove 1 µL of the purified captured library pool from the sample and
combine with 9 µL of 1 X Low TE Buffer for Bioanalyzer analysis.
Stopping Point If you do not continue to the next step, seal the plate and store at 4°C
overnight or at –20°C for prolonged storage.