Technical data
63
SureSelect
XT2
Target Enrichment System for Illumina Multiplexed
Sequencing Protocol
Agilent Technologies
5
Post-Capture Sample Processing for
Multiplexed Sequencing
Step 1. Amplify the captured libraries 64
Step 2. Purify the amplified captured libraries using AMPure XP beads 66
Step 3. Assess quality with the 2100 Bioanalyzer High Sensitivity DNA
assay 68
Step 4. Prepare samples for multiplexed sequencing 70
Step 5. Optional: Quantify captured library pools by QPCR 72
Step 6. Optional: Pool captured libraries for sequencing 73
This chapter describes the steps to amplify, purify, and assess quality and
quantity of the captured libraries. Post-capture dilution and optional
pooling instructions are provided to prepare the indexed samples for
multiplexed sequencing.