Technical data

Hybridization 4
Step 4. Capture the hybridized DNA using streptavidin beads
SureSelect
XT2
Target Enrichment System for Illumina 61
10 W
ash the beads with SureSelect XT2 Wash 2:
a Resuspend t
he beads in 200 µL of 65°C prewarmed SureSelect XT2
Wash 2. Pipette up and down until the beads are fully resuspended.
b Incubat
e the sample plate for 5 minutes at 65°C on the SureCycler
thermal cycler.
c Brief
ly spin the plate in a centrifuge or mini-plate spinner.
d Put the plate in the magnetic separator.
e Wait for the solution to clear, then remove and discard the
supernatant.
f Repeat step a through step e for a total of 6 washes.
Make sure all of the wash buffer has been removed during the final
wash.
11 Mix the beads in each well with 30 µL of nuclease-free water on a
vortex mixer for 5 seconds to resuspend the beads.
Captured DNA is retained on the streptavidin beads during the post-capture amplification
step.
Stopping Point If you do not continue to the next step, seal the plate and store at 4°C
overnight or at –20°C for prolonged storage.
NOTE