Technical data

Hybridization 4
Step 3. Prepare streptavidin-coated magnetic beads
SureSelect
XT2
Target Enrichment System for Illumina 59
Step 3. Prepare streptavidin-coated magnetic beads
1 Prewarm SureSelect XT2 Wash 2 at 65°C in a water bath or heat block
for use in “Step 4. Capture the hybridized DNA using streptavidin
beads”.
2 Vigor
ously resuspend the Dynabeads MyOne Streptavidin T1 magnetic
beads on a vortex mixer. The magnetic beads settle during storage.
3 For each hybridization sample, add 50 µL of the resuspended beads to
wells of a SureCycler 8800 PCR plate.
4 Wash the beads:
a Add 200 µL of SureSelect XT2 Binding Buffer.
b Mix by pipetting up and down until the beads are fully resuspended.
c Put the plate into a magnetic separator device.
d Wait for the solution to clear, then remove and discard the
supernatant.
e Repeat step a through step d for a total of 3 washes.
5 Resuspend the beads in 200 µL of SureSelect XT2 Binding Buffer.
For runs that include multiple sample capture wells, the streptavidin beads may be
batch-washed in an Eppendorf tube or conical vial. Start the batch-washing procedure
using excess bead solution. After resuspending the washed beads in the appropriate
volume of SureSelect Binding Buffer, aliquot 200 l of the washed beads to each well to be
used for hybridization capture.
NOTE