Technical data

3 Sample Preparation (100 ng DNA Samples)
Step 10. Assess quality with the 2100 Bioanalyzer DNA 1000 Assay
52 SureSelect
XT2
Target Enrichment System for Illumina
Step 10. Assess quality with the 2100 Bioanalyzer DNA 1000
Assay
You can use Agilent’s 2200 TapeStation for rapid analysis of multiple samples at this step.
For analysis of indexed DNA amplicons prepared from 100-ng gDNA samples, use the
D1000 ScreenTape (p/n 5067-5582) and associated reagents. See the 2200 TapeStation and
D1000 ScreenTape protocols for information on sample preparation and data analysis.
Use a Bioanalyzer DNA 1000 chip and reagent kit for analysis of indexed
DNA amplicons prepared from 100-ng gDNA samples. See the DNA 1000
Kit Guide at www.genomics.agilent.com for more information on doing this
step.
1 Check that the 2100 Bioanalyzer electrodes have been cleaned as
instructed in the reagent kit guide.
2 Open the 2100 Expert Software (version B.02.07 or higher), turn on the
2100 Bioanalyzer and check communication.
3 Prepare the chip, samples and ladder as instructed in the reagent kit
guide, using 1 µL of each sample for the analysis.
4 Load the prepared chip into the 2100 Bioanalyzer and start the run
within five minutes after preparation.
5 Within the instrument context, choose the DNA 1000 assay from the
drop down list.
6 Start the run. Enter sample names and comments in the Data and
Assay context.
7 Verify that the electropherogram shows a distribution with an average
DNA fragment size of approximately 250 to 275 bp. A sample
electropherogram is shown in Figure 5.
8 Measure the concentration of the library by integrating under the peak.
For accurate quantification, make sure that the concentration falls
within the linear range of the assay.
After determining the DNA concentration for each sample, proceed to
“Hybridization” on page 55.
NOTE