Technical data

SureSelect
XT2
Target Enrichment System for Illumina 5
What’s New in Version E.0
• Support for kits supplied with either of two indexing
primer configurations.
Kits with revised index configuration (typically received
February 2015 or later) include indexing primers A01
through H02 provided in white-capped tubes (16 Reaction
kits) or indexing primers A01 through H12 provided in a
blue plate (96 Reaction kits). For kit content details see
page 76. For nucleotide sequences of the 8-bp indexes in
this revised configuration, see Table 38 on page 79.
Kits with original index configuration (typically received
before February 2015), include indexing primers 1–16 or
1–96 provided in clear-capped tubes. For kit content
details see page 80. For nucleotide sequences of the 8-bp
indexes in this original configuration, see Table 44 on
page 82 through Table 49 on page 87.
• Support for revised Library Prep kit configuration, now
including End Repair Enzyme Mix and End Repair Oligo
Mix (both replacing End Repair Master Mix). For
protocols for use of the revised kits, see page 25 for 1 g
DNA samples or see page 43 for 100 ng DNA samples.
See Table 34 on page 77 for updated kit contents.
• Updates to solvents used in the Sample Preparation
protocol for 100 ng DNA samples. Updates include initial
dilution of gDNA samples in 1X Low TE Buffer (step 2 on
page 38), and DNA elution from AMPure XP beads using
nuclease-free water (step 13 on page 46 and step 12 on
page 49).
• Update to instructions for dilution of pre-capture
indexing adaptors in the Sample Preparation protocol for
100 ng DNA samples. See step 1 on page 48.
• Update to instructions for washing streptavidin-coated
magnetic beads. See step 4 on page 59.