Technical data

3 Sample Preparation (100 ng DNA Samples)
Step 6. Ligate the pre-capture indexing adaptor
48 SureSelect
XT2
Target Enrichment System for Illumina
Step 6. Ligate the pre-capture indexing adaptor
See the Reference section for sequences of the index portion of the
indexing adaptors that are ligated to gDNA libraries in this section.
Be sure to keep the sample plate at 4°C or on ice while doing step 1
through step 3 sequentially as outlined below.
1 F
or each of the SureSelect
XT2
Pre-capture Index solutions to be used in
the run, prepare a 1:5 dilution in nuclease-free water.
2 Add 5 µL of SureSelect Ligation Master Mix to each A-tailed DNA
sample.
3 Using the diluted indexing adaptor solutions prepared in step 1 above,
add 5 µL of the appropriate index dilution to each sample.
4 Seal the wells then mix thoroughly by vortexing for 5 seconds. Briefly
spin the plate, then keep the plate on ice until it is placed in the
thermal cycler in step 5.
5 Incubate the plate in the SureCycler thermal cycler and run the
program in Table 20. Do not use a heated lid.
Table 20 Ligation Thermal Cycler Program
CAUTION
SureSelect
XT2
master mixes are viscous and thorough mixing is required to combine
these mixtures with other solutions.
Step Temperature Time
Step 1 20°C 15 minutes
Step 2 4°C Hold