Technical data

Sample Preparation (100 ng DNA Samples) 3
Step 5. Adenylate the 3' end of the DNA fragments
SureSelect
XT2
Target Enrichment System for Illumina 47
Step 5. Adenylate the 3' end of the DNA fragments
1 Add 20 µL of SureSelect dA-Tailing Master Mix to each end-repaired,
purified DNA sample (approximately 20 µL).
2 Mix w
ell by pipetting up and down or by gentle vortexing.
3 Incubate the plate in the SureCycler thermal cycler and run the
program in Table 19. Do not use a heated lid.
Table 19 dA-Tailing Thermal Cycler Program
The thermal cycling program for dA-Tailing of 100 ng samples differs from the 1 g-input
dA-Tailing thermal cycling program. Be sure to include the 10-minute incubation at 60°C
when preparing 100 ng DNA samples.
CAUTION
SureSelect
XT2
master mixes are viscous and thorough mixing is required to combine
these mixtures with other solutions.
Step Temperature Time
Step 1 37°C 30 minutes
Step 2 60°C 10 minutes
Step 3 4°C Hold
NOTE