Technical data

3 Sample Preparation (100 ng DNA Samples)
Step 4. Purify the sample using AMPure XP beads
46 SureSelect
XT2
Target Enrichment System for Illumina
11 Seal the wells with strip caps, then briefly spin the plate to collect the
residual ethanol. Return the plate to the magnetic stand for 30 seconds.
Remove the residual ethanol with a P20 pipette.
12 Dry the samples on the SureCycler thermal cycler, set to hold samples
at 37°C, for 5 to 10 minutes or until the residual ethanol completely
evaporates.
13 Add 22 µL nuclease-free water to each sample well.
14 Seal the wells with strip caps, then mix well on a vortex mixer and
briefly spin the plate in a centrifuge or mini-plate spinner to collect the
liquid.
15 Incubate for 2 minutes at room temperature.
16 Put the plate in the magnetic stand and leave for 2 to 3 minutes, until
the solution is clear.
17 Remove the cleared supernatant to a fresh SureCycler 8800 PCR plate
well. You can discard the beads at this time.
Stopping Point If you do not continue to the next step, seal the plate and store at –20°C.