Technical data

Sample Preparation (100 ng DNA Samples) 3

Step 4. Purify the sample using AMPure XP beads

SureSelect

XT2

Target Enrichment System for Illumina 45

Step 4. Purify the sample using AMPure XP beads

1 Let the AMPure XP beads come to room temperature for at least

30 minutes. Do not freeze the beads at any time.

2 Prepare 400 µL of 70% et

hanol per sample, plus excess, for use in

step 8.

The freshly-prepared 70% ethanol may be used for subsequent purification steps run on the

same day

. The complete Library Preparation protocol requires 1.2 mL of fresh 70% ethanol

per sample.

3 Mix the bead suspension well so that the reagent appears homogeneous

and consistent in color.

4 Add 180 µL of homogeneous AMPure XP beads to each 100-µL

end-repaired DNA library sample in the PCR plate. Pipette up and

down 10 times to mix.

5 Incubate samples for 5 minutes at room temperature.

6 Put the plate into a magnetic separation device, such as the Dynal

magnetic separator. Wait for the solution to clear (approximately 3 to

5 minutes).

7 Keep the plate in the magnetic stand. Carefully remove and discard the

cleared solution from each well. Do not touch the beads while removing

the solution.

8 Continue to keep the plate in the magnetic stand while you dispense

200 µL of 70% ethanol in each sample well.

Use fresh 70% ethanol for optimal results.

9 Wait for 1 minute to allow any disturbed beads to settle, then remove

the ethanol.

10 Repeat step 8 and step 9 step once.

NOTE

If some magnetic beads remain suspended in solution after 5 minutes, carefully remove and

discard 100 l of cleared solution from near the bottom of the tube, and continue

incubating the tube in the magnetic stand for an additional 3 minutes. After the remaining

suspension has cleared, remove and discard the remaining cleared solution (approximately

180 l) from the well.