Technical data

Sample Preparation (100 ng DNA Samples) 3
Step 3. Repair the ends
SureSelect
XT2
Target Enrichment System for Illumina 43
Step 3. Repair the ends
Use the SureSelect
XT2
Library Prep Kit.
Hold samples on ice during the following steps.
Instructions for Library Prep Kit p/n 5500-0130 or 5500-0131
1 Prepare the appropriate volume of End Repair Reaction Mix, as
described in Table 16, on ice. Mix well on a vortex mixer.
Table 16 Preparation of SureSelect End Repair Reaction Mix
2 To each 50-µL sheared DNA sample well, add 50 µL of the SureSelect
End Repair Reaction Mix prepared in step 1.
3 Mix well by pipetting up and down or by gentle vortexing.
CAUTION
This section contains two versions of instructions for the end repair reaction. The first
section covers kits supplied with Library Prep Kit p/n 5500-0130 or 5500-0131, with two
separate reagent vials containing the End Repair Enzyme Mix and End Repair Oligo Mix
(typically received February, 2015 or later).
The second section covers kits supplied with Library Prep Kit p/n 5500-0102 or
5500-0103, with pre-combined End Repair Master Mix (typically received before
February, 2015).
Verify that you are referencing the information appropriate for your kit version before
you proceed.
Reagent Volume for 1
reaction
Volume for 16 reactions
(includes excess)
SureSelect End Repair Enzyme Mix 40 µL 660 µL
SureSelect End Repair Oligo Mix 10 µL 165 µL
Total 50 µL 825 µL