Technical data

Sample Preparation (100 ng DNA Samples) 3
Step 2. Assess quality (optional)
SureSelect
XT2
Target Enrichment System for Illumina 41
Step 2. Assess quality (optional)
You can use Agilent’s 2200 TapeStation for rapid analysis of multiple samples at this step.
For analysis of 100 ng sheared DNA samples, use the High-Sensitivity D1000 ScreenTape
(p/n 5067-5584) and associated reagents. See the 2200 TapeStation and High-Sensitivity
D1000 ScreenTape protocols for information on sample preparation and data analysis.
This step is optional.
Use a High Sensitivity DN
A Assay kit for analysis of the 100 ng sheared
DNA samples using the 2100 Bioanalyzer. See the High Sensitivity DNA
Kit Guide at www.genomics.agilent.com, for more information on doing
this step.
1 Check that the 2100 Bioanalyzer electrodes have been cleaned as
instructed in the reagent kit guide.
2 Open the 2100 Expert Software (version B.02.07 or higher required to
run the High Sensitivity Kit), turn on the 2100 Bioanalyzer, and check
communication.
3 Prepare the chip, samples and ladder as instructed in the reagent kit
guide, using 1 µL of each sheared DNA sample for the analysis.
4 Load the prepared chip into the 2100 Bioanalyzer and start the run
within five minutes after preparation.
5 Within the instrument context, choose the High Sensitivity DNA assay
from the drop down list.
6 Start the run. Enter sample names and comments in the Data and
Assay context.
7 Check that the electropherogram shows an average DNA fragment size
of 150 to 200 bp. A sample electropherogram is shown in Figure 4.
NOTE