Technical data

4 SureSelect
XT2
Target Enrichment System for Illumina
In this Guide...
This guide describes an optimized protocol for Illumina
paired-end multiplexed library preparation using the
SureSelect
XT2
Library Prep and Capture System.
This protocol is specifically optimized to enrich targeted
regions of the genome from repetitive sequences and
sequences unrelated to the research focus prior to sample
sequencing using the Illumina platform. The SureSelect
XT2
Library Prep and Capture System features pre-capture
indexing reagents and protocols.
1 Before You Begin
This chapter contains information (such as procedural notes,
safety information, required reagents and equipment) that
you should read and understand before you start an
experiment.
2 Sample Preparation (1 µg DNA Samples)
This chapter describes the steps to prepare index-tagged
libraries for target enrichment from 1-µg gDNA samples.
3 Sample Preparation (100 ng DNA Samples)
This chapter describes the steps to prepare index-tagged
libraries for target enrichment from 100-ng gDNA samples.
4 Hybridization
This chapter describes the steps to pool indexed libraries
and then hybridize and capture the pooled DNA.
5 Post-Capture Sample Processing for Multiplexed Sequencing
This chapter describes the steps to amplify, purify, and
assess quality and quantity of the sample libraries. Samples
are pooled by mass prior to sequencing.
6 Reference
This chapter contains reference information, including
component kit contents and index sequences.