Technical data

3 Sample Preparation (100 ng DNA Samples)

Step 1. Shear DNA

38 SureSelect

XT2

Target Enrichment System for Illumina

Step 1. Shear DNA

Make sure genomic DNA samples are of high quality with an OD 260/280 ratio ranging

from 1.8 to 2.0.

For each DNA sample to be sequenced, prepare 1 library.

1 Use t

he Qubit dsDNA BR Assay to determine the concentration of your

gDNA sample.

Follow the instructions for the instrument.

2 Dilute 100 ng of high-quality gDNA with 1X Low TE Buffer in a 1.5-mL

LoBind tube to a total volume of 50 µL.

3 Set up the Covaris E-series or S-series instrument.

a Check that the water in the Covaris tank is filled with fresh

deionized water to the appropriate fill line level according to the

manufacturer’s recommendations for the specific instrument model

and sample tube or plate in use.

b Check that the water covers the visible glass part of the tube.

c On the instrument control panel, push the Degas button. Degas the

instrument for least 2 hours before use, or according to the

manufacturer’s recommendations.

d Set the chiller temperature to between 2°C to 5°C to ensure that the

temperature reading in the water bath displays 5°C.

e Optional. Supplement the circulated water chiller with ethylene

glycol to 20% volume to prevent freezing.

Refer to the Covaris instrument user guide for more details.

4 Put a Covaris microTube into the loading and unloading station.

Keep the cap on the tube.

You can use the 96 microTube plate (see Table 3 on page 16) for the DNA shearing step

when preparing multiple gDNA samples in the same experiment.

5 Use a tapered pipette tip to slowly transfer the 50-µL DNA sample

through the pre-split septa.

Be careful not to introduce a bubble into the bottom of the tube.

NOTE