Technical data
Sample Preparation (1 µg DNA Samples) 2
Step 8. Amplify the indexed library
SureSelect
XT2
Target Enrichment System for Illumina 33
3 Run t
he program in Table 13 in a SureCycler thermal cycler.
Different library preparations can produce slightly different results, based on varying DNA
quality
. In most cases, five cycles will produce an adequate yield for subsequent capture
without introducing bias or non-specific products. If yield is too low, or too high (where
non-specific high molecular weight products are observed), adjust the number of cycles
accordingly to amplify the remaining indexed library.
.
Table 13 Pre-Capture PCR Thermal Cycler Program
Segment Number of
Cycles
Temperature Time
1 1 98°C 2 minutes
2 5 98°C 30 seconds
60°C 30 seconds
72°C 1 minute
3 1 72°C 10 minutes
4 1 4°C Hold
NOTE