Technical data

2 Sample Preparation (1 µg DNA Samples)
Step 8. Amplify the indexed library
32 SureSelect
XT2
Target Enrichment System for Illumina
Step 8. Amplify the indexed library
This protocol uses half of the indexing adaptor-ligated library for
amplification. The remainder can be saved at –20°C for future use, if
needed.
1 Prepare the appropriate volume of pre-capture PCR reaction mix, as
described in Table 12, on ice. Mix well on a vortex mixer.
2 In separate wells of a SureCycler 8800 PCR plate, combine 26 µL of the
amplification mixture prepared in Table 12 and 24 µL of each indexed
gDNA library sample.
Mix by pipetting. Change pipette tips between samples.
CAUTION
To avoid cross-contaminating libraries, set up PCR reactions (all components except
the library DNA) in a dedicated clean area or PCR hood with UV sterilization and
positive air flow.
Table 12 Preparation of SureSelect Pre-Capture PCR Reaction Mix
Reagent Volume for 1
reaction
Volume for 16 reactions
(includes excess)
XT2 Primer Mix 1 µL 16.5 µL
Herculase II PCR Master Mix 25 µL 412.5 µL
Total 26 µL 429 µL