Technical data

2 Sample Preparation (1 µg DNA Samples)
Step 6. Ligate the pre-capture indexing adaptor
30 SureSelect
XT2
Target Enrichment System for Illumina
Step 6. Ligate the pre-capture indexing adaptor
See the Reference section for sequences of the index portion of the
indexing adaptors that are ligated to gDNA libraries in this section.
Be sure to keep the sample plate at 4°C or on ice while doing step 1 and
step 2 sequentially as outlined below.
1 A
dd 5 µL of SureSelect Ligation Master Mix to each A-tailed DNA
sample.
2 Add 5 µL of the appropriate SureSelect
XT2
Pre-capture Index solution
to each sample.
3 Seal the wells then mix thoroughly by vortexing for 5 seconds. Briefly
spin the plate, then keep the plate on ice until it is placed in the
thermal cycler in step 4.
4 Incubate the plate in the SureCycler thermal cycler and run the
program in Table 11. Do not use a heated lid.
Table 11 Ligation Thermal Cycler Program
CAUTION
SureSelect
XT2
master mixes are viscous and thorough mixing is required to combine
these mixtures with other solutions.
Step Temperature Time
Step 1 20°C 15 minutes
Step 2 4°C Hold