Technical data

2 Sample Preparation (1 µg DNA Samples)
Step 3. Repair the ends
26 SureSelect
XT2
Target Enrichment System for Illumina
4 Incubat
e the plate in the SureCycler thermal cycler and run the
program in Table 8. Do not use a heated lid.
Table 8 End-Repair Thermal Cycler Program
Instructions for Library Prep Kit p/n 5500-0102 or 5500-0103
1 Add 50 µL of SureSelect End Repair Master Mix to each sheared DNA
sample.
2 Mix well by pipetting up and down or by gentle vortexing.
3 Incubate the plate in the SureCycler thermal cycler and run the
program in Table 8. Do not use a heated lid.
Table 9 End-Repair Thermal Cycler Program
Step Temperature Time
Step 1 20°C 30 minutes
Step 2 4°C Hold
CAUTION
SureSelect
XT2
master mixes are viscous and thorough mixing is required to combine
these mixtures with other solutions.
Step Temperature Time
Step 1 20°C 30 minutes
Step 2 4°C Hold