Technical data

Sample Preparation (1 µg DNA Samples) 2
Step 2. Assess quality (optional)
SureSelect
XT2
Target Enrichment System for Illumina 23
Step 2. Assess quality (optional)
You can use Agilent’s 2200 TapeStation for rapid analysis of multiple samples at this step.
For analysis of 1 g sheared DNA samples, use the D1000 ScreenTape (p/n 5067-5582) and
associated reagents. See the 2200 TapeStation and D1000 ScreenTape protocols for
information on sample preparation and data analysis.
This step is optional.
Use a Bioanal
yzer DNA 1000 chip and reagent kit for analysis of the 1 g
sheared DNA samples using the 2100 Bioanalyzer. See the DNA 1000 Kit
Guide at www.genomics.agilent.com for more information on doing this
step.
1 Check that the 2100 Bioanalyzer electrodes have been cleaned as
instructed in the reagent kit guide.
2 Open the 2100 Expert Software (version B.02.07 or higher), turn on the
2100 Bioanalyzer and check communication.
3 Prepare the chip, samples and ladder as instructed in the reagent kit
guide, using 1 µL of each sample for the analysis.
4 Load the prepared chip into the 2100 Bioanalyzer and start the run
within five minutes after preparation.
5 Within the instrument context, choose the DNA 1000 assay from the
drop down list.
6 Start the run. Enter sample names and comments in the Data and
Assay context.
7 Check that the electropherogram shows an average DNA fragment size
of 150 to 200 bp. A sample electropherogram is shown in Figure 2.
NOTE