Technical data

Sample Preparation (1 µg DNA Samples) 2
Step 1. Shear DNA
SureSelect
XT2
Target Enrichment System for Illumina 21
6 Secure the microTube in the tube holder and shear the DNA with the
settings in Table 5 or Table 6, depending on the Covaris instrument
SonoLab software version used.
The target DNA fragment size is 150 to 200 bp.
7 Put the Covaris microTube back into the loading and unloading station.
8 While keeping the snap-cap on, insert a pipette tip through the
pre-split septa, then slowly remove the sheared DNA.
Table 5 Shear settings for Covaris instruments using SonoLab software version 7 or
newer
Setting Value
Duty Factor 10%
Peak Incident Power (PIP) 175
Cycles per Burst 200
Treatment Time 360 seconds
Bath Temperature 4° to 8° C
Table 6 Shear settings for Covaris instruments using SonoLab software prior to
version 7
Setting Value
Duty Cycle 10%
Intensity 5
Cycles per Burst 200
Time 6 cycles of 60 seconds each
Set Mode Frequency sweeping
Temperature 4° to 7° C