Technical data

Reference 6
Alternative Capture Equipment Combinations
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 97
Alternative Capture Equipment Combinations
Table 58 below lists combinations of thermal cyclers, lid temperatures,
plates or strip tubes and sealing methods that have shown minimal
evaporation when used for the Hybridization protocol on page 60.
Refer to this list for additional equipment and plasticware combination
options for hybridization, beyond the combinations used for protocol
optimization and supported by Agilent. Note that minimal evaporation is
required to ensure optimal capture results.
Table 58 Tested options that show minimal evaporation
PCR Machine Plate/Strips Cover Comments
Agilent Mx3005P Real-Time PCR
System
Mx3005P Strip Tubes
(Agilent p/n 401428)
Mx3005P Optical Strip Caps
(Agilent p/n 401425)
Heated lid
Agilent Mx3005P Real-Time PCR
System
Life Technologies ABI
MicroAmp Optical 96-well
plates (p/n N8010560)
MicroAmp clear adhesive
film (p/n 4306311)
Heated lid; use ABI
compression pad (4312639);
use two layers of film
ABI GeneAmp 9700 Life Technologies ABI
MicroAmp Optical 96-well
plates (p/n N8010560)
MicroAmp caps (p/n
N8010535)
Heated lid
ABI Veriti (p/n 4375786) Life Technologies ABI
MicroAmp Optical 96-well
plates (p/n N8010560)
MicroAmp clear adhesive
film (p/n 4306311)
Heated lid; use ABI
compression pad (4312639);
use two layers of film
Eppendorf Mastercycler Eppendorf 8-Tube PCR
Tubes
Attached caps Lid heating set to 75°C
BioRad (MJ Research) PTC-200 Mx4000 Strip Tubes
(Agilent p/n 410022)
Mx4000 Optical Caps
(Agilent p/n 401024)
Heated lid
BioRad (MJ Research) PTC-200 Mx4000 Strip Tubes
(Agilent p/n 410022)
Mx3005P Optical Strip Caps
(Agilent p/n 401425)
Heated lid
BioRad (MJ Research) PTC-200 Mx3005P 96-well plate
(Agilent p/n 410088)
Mx3005P Optical Strip Caps
(Agilent p/n 401425)
Heated lid