Technical data

SureSelect

Target Enrichment System for Illumina Paired-End

Sequencing Library Protocol

Agilent Technologies

Before You Begin

Overview of the Workflow 10

Procedural Notes 12

Safety Notes 13

Required Reagents 14

Optional Reagents 16

Required Equipment 17

Optional Equipment 19

Make sure you have the most current protocol. Go to genomics.agilent.com

and search for G7530-90000.

This protocol differs from the Illumina Multiplexed Paired-End sequencing manual and

other SureSelect protocols at several steps. Pay close attention to the primers used for

each amplification step and the blocking agents used during hybridization.

Agilent cannot guarantee the SureSelect Target Enrichment kits and cannot provide

technical support for the use of non-Agilent protocols or instruments to process samples

for enrichment.

Make sure you read and understand the information in this chapter and

have the necessary equipment and reagents listed before you start an

experiment.

NOTE