Technical data

Indexing and Sample Processing for Multiplexed Sequencing 5
Step 5. Pool samples for multiplexed sequencing
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 85
• If the final volume of the combined index-tagged samples is less than
the desired final volume, V(f), add Low TE to bring the volume to
the desired level.
• If the final volume of the combined index-tagged samples is greater
than the final desired volume, V(f), lyophilize and reconstitute to the
desired volume.
3 If you store the library before sequencing, add Tween 20 to 0.1% v/v
and store at -20°C short term.