Technical data

Indexing and Sample Processing for Multiplexed Sequencing 5
Step 1A. Amplify the captured libraries with indexing primers containing 8-bp indexes A01–H12
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 73
7 When the PCR amplification program is complete, spin the plate or
strip tube briefly.
Proceed to “Step 2. Purify the amplified captured libraries using
AMPure XP beads” on page 77.
Table 40 Post-capture PCR cycle number recommendations
Capture Size Cycles
1 kb up to 0.5 Mb 16 cycles
0.5 Mb up to 1.49 Mb 14 cycles
>1.5 Mb 12 cycles
All Exon and Exome libraries 10 to 12 cycles
OneSeq libraries (all designs) 10 cycles