Technical data

4 Hybridization and Capture
Step 3. Capture the hybridized DNA using streptavidin-coated beads
68 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
10 Wash the beads with SureSelect Wash Buffer 2:
a Resuspend the beads in 200 µL of 65°C prewarmed Wash Buffer 2.
Pipette up and down until beads are fully resuspended.
b Cap the wells, then incubate the sample plate or strip tube for
10 minutes at 65°C on the thermal cycler.
c Put the plate or strip tube in the magnetic separator. Wait for the
solution to clear, then remove and discard the supernatant.
d Repeat step a through step c for a total of 3 washes.
Make sure all of the wash buffer has been removed during the final
wash.
11 Add 30 µL of nuclease-free water to each sample well. Pipette up and
down to resuspend the beads.
Keep the samples on ice until they are used on page 71.
Captured DNA is retained on the streptavidin beads during the post-capture amplification
step.
CAUTION
It is important to maintain bead suspensions at 65°C during the washing procedure
below to ensure specificity of capture.
Make sure that the SureSelect Wash Buffer 2 is pre-warmed to 65°C before use.
Do not use a tissue incubator, or other devices with significant temperature
fluctuations, for the incubation steps.
NOTE