Technical data

Hybridization and Capture 4
Step 1. Hybridize DNA samples to the Capture Library
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 65
10 Maintain the gDNA library + Block Mix plate or strip tube at 65°C
while you add 20 µL of the Capture Library Hybridization Mix from
step 9 to each sample well. Mix well by pipetting up and down 8 to 10
times.
The hybridization reaction wells now contain approximately 27 to
29 µL, depending on the degree of evaporation during the thermal
cycler incubation.
11 Seal the wells with strip caps or using the PlateLoc Thermal Microplate
Sealer. Make sure that all wells are completely sealed.
12 Incubate the hybridization mixture for 16 or 24 hours at 65°C with a
heated lid at 105°C.
CAUTION
Wells must be adequately sealed to minimize evaporation, or your results can be
negatively impacted.
When using the SureCycler 8800 thermal cycler and sealing with strip caps, make sure
to use domed strip caps and to place a compression mat over the PCR plate or strip
tubes in the thermal cycler.