Technical data

4 Hybridization and Capture
Step 1. Hybridize DNA samples to the Capture Library
64 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
9 Prepare the Capture Library Hybridization Mix appropriate for your
Capture Library size according to Table 35 (Capture Libraries3 Mb), or
Table 36 (Capture Libraries<3 Mb).
Mix well by vortexing at high speed for 5 seconds then spin down
briefly. Keep the mixture at room temperature briefly, until use in
step 10.
Table 35 Preparation of Capture Library Hybridization Mix for 3 Mb Capture Libraries
Table 36 Preparation of Capture Library Hybridization Mix for <3 Mb Capture Libraries
NOTE
Prepare the Capture Library mixture described in step 9, below, near the end of the 65°C
hold step of >5 minute duration) described in Table 33. Keep the mixture at room
temperature briefly, until adding the mixture to sample wells in step 10. Do not keep
solutions containing the Capture Library at room temperature for extended periods.
Reagent Volume for 1 reaction Volume for 16 reactions
(includes excess)
Hybridization Buffer mixture from step 4 13 µL 221 µL
25% RNase Block solution from step 8 2 µL 34 µL
Capture Library
3 Mb 5 µL 85 µL
Total 20 µL 340 µL
Reagent Volume for 1 reaction Volume for 16 reactions
(includes excess)
Hybridization Buffer mixture from step 4 13 µL 221 µL
10% RNase Block solution from step 8 5 µL 85 µL
Capture Library <3 Mb 2 µL 34 µL
Total 20 µL 340 µL