Technical data

4 Hybridization and Capture
Step 1. Hybridize DNA samples to the Capture Library
62 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
4 Prepare t
he Hybridization Buffer by mixing the components in Table 31
at room temperature.
If a precipitate forms, warm the Hybridization Buffer at 65°C for
5 minutes.
Keep the prepared Hybridization Buffer at room temperature until it is
used in step 9.
Table 31 Preparation of Hybridization Buffer
5 Prepare the SureSelect Block Mix by mixing the components in
Table 32. Keep the mixture on ice until it is used in step 6.
Table 32 Preparation of SureSelect Block Mix
Reagent Volume for 1 reaction
*
* Prepare Hybridization Buffer for at least 5 reaction equivalents per run to allow accurate pipetting
volumes.
Volume for 16 reactions
(includes excess)
SureSelect Hyb 1 (orange cap or bottle) 6.63 µL 116 µL
SureSelect Hyb 2 (red cap) 0.27 µL 4.7 µL
SureSelect Hyb 3 (yellow cap or bottle) 2.65 µL 46.4 µL
SureSelect Hyb 4 (black cap or bottle) 3.45 µL 60.4 µL
Total 13 µL 227.5
Reagent Volume for 1 reaction Volume for 16 reactions
(includes excess)
SureSelect Indexing Block 1 (green cap) 2.5 µL 42.5 µL
SureSelect Block 2 (blue cap) 2.5 µL 42.5 µL
SureSelect ILM Indexing Block 3 (brown cap) 0.6 µL 10.2 µL
Total 5.6 µL 95.2 µL