Technical data

4 Hybridization and Capture
Step 1. Hybridize DNA samples to the Capture Library
60 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
Step 1. Hybridize DNA samples to the Capture Library
In this step, the prepared gDNA libraries are hybridized to a
target-specific Capture Library.
This step uses the SureSelect
XT
Reagent Kit components listed in Table 30.
Thaw each component under the conditions indicated in the table. Vortex
each reagent to mix, then spin tubes briefly to collect the liquid.
Table 30 Reagents for Hybridization
Kit Component Storage Location Thawing Conditions Where Used
SureSelect Hyb 1 SureSelect Target Enrichment-Box 1, RT — page 62
SureSelect Hyb 2 SureSelect Target Enrichment-Box 1, RT — page 62
SureSelect Hyb 3 SureSelect Target Enrichment Kit ILM
Indexing Hyb Module Box 2, –20°C
Warm to Room
Temperature (RT)
page 62
SureSelect Hyb 4 SureSelect
Target Enrichment-Box 1, RT — page 62
SureSelect Indexing Block 1 SureSelect Target Enrichment Kit ILM
Indexing Hyb Module Box 2, –20°C
Thaw on ice page 62
SureSelect Block 2 SureSelect Target Enrichment Kit ILM
Indexing Hyb Module Box 2, –20°C
Thaw on ice page 62
SureSelect ILM Indexing Block 3 SureSelect Target Enrichment Kit ILM
Indexing Hyb Module Box 2, –20°C
Thaw on ice page 62
SureSelect RNase Block SureSelect Target Enrichment Kit ILM
Indexing Hyb Module Box 2, –20°C
Thaw on ice page 63
Capture Library –80°C Thaw on ice page 64